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Effect of Allelic Variants of Aromatic Alcohol Dehydrogenase CADim on Micromorphological and Chemical Tissue Indices in the Spring Bread Wheat Triticum aestivum L.

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Abstract

The inheritance of allelic variants of aromatic alcohol dehydrogenase CADim (CAD intermediate) and their effect on the severity of plant traits in spring bread wheat (fluorescence of seedling slices, micromorphology, and chemical composition of tissues) were studied. The plants with contrast CADim genotypes (homozygotes +/+ and –/–) were used to study the tissue autofluorescence, micromorphology, and chemical composition. The thickness of the straw walls in the CADim– genotype is higher than that in the CADim+ genotype, which can affect the resistance of wheat plants to lodging. Differences in the chlorophyll content, particularly, in the ratio of chlorophylls A and B, were observed; this probably affects photosynthesis. An increased content of carbonyl groups in the CADim+ genotype, as well as differences in the cinnamon monomers of lignin, were found. It follows from these results that CADim+ and CADim– allelic variants have a significant effect on a number of plant traits and, consequently, that the polymorphism at the CADim locus is functional, which enables its use in breeding and biotechnology.

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ACKNOWLEDGMENTS

Autofluorescence was studied on cross sections of weekly seedlings AT the Center for Collective Use for Microscopic Analysis of Biological Objects of the Siberian Branch of the Russian Academy of Sciences. Spectral and analytical measurements were conducted at the Multi-Access Chemical Research Center of the Siberian Branch of the Russian Academy of Sciences.

Funding

This work was supported by budget projects nos. 0259-2021-0012 (Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences) and 0302-2020-0005 (Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences), as well as by the Russian Foundation for Basic Research (regional project no. 19-44-540003 r_a).

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Authors and Affiliations

  1. Federal Research Center Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    A. A. Konovalov & N. P. Goncharov

  2. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    E. V. Karpova & I. K. Shundrina

  3. Siberian Research Institute of Plant Cultivation and Breeding—Branch of Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630501, Krasnoobsk, Novosibirsk region, Russia

    E. P. Razmakhnin

  4. Novosibirsk State University, 630090, Novosibirsk, Russia

    I. V. Eltsov

  5. Novosibirsk State Agrarian University, 630039, Novosibirsk, Russia

    N. P. Goncharov

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  1. A. A. Konovalov
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  2. E. V. Karpova
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  3. I. K. Shundrina
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Correspondence to A. A. Konovalov.

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Translated by A. Barkhash

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Konovalov, A.A., Karpova, E.V., Shundrina, I.K. et al. Effect of Allelic Variants of Aromatic Alcohol Dehydrogenase CADim on Micromorphological and Chemical Tissue Indices in the Spring Bread Wheat Triticum aestivum L.. Appl Biochem Microbiol 57, 521–532 (2021). https://doi.org/10.1134/S0003683821040086

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