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Optimization of Microbial Synthesis of Silver Sulfide Nanoparticles

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Abstract

The method for the biosynthesis of silver sulfide nanoparticles using a strain of Shewanella oneidensis MR-1 in an aqueous solution of silver nitrate and sodium thiosulfate salts has been optimized. Optimization is associated with the biosynthesis of nanoparticles directly in the culture medium in the presence of live cells, which increases the nanoparticle yield by 15–20% and significantly shortens the entire process of obtaining nanoparticles. It was also shown that an increase in the concentration of sodium thiosulfate and silver nitrate salts from 1 mM to 10 mM increases the nanoparticle concentration in the reaction solution, whereas the nanoparticle yield in terms of the silver introduced in the reaction decreases. The nanoparticles obtained by the optimized and the conventional methods do not differ in shape, size, and chemical composition. It was shown that the efficiency of nanoparticle biosynthesis depends on the composition of the liquid nutrient medium for S. oneidensis MR-1 cell growth. Cell culturing in nutrient medium for over 24 h fails to intensify the biosynthesis process.

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ACKNOWLEDGMENTS

The work was financially supported by the Russian Fund for Basic Research, project no. 16-04-00471. The electron microscopy assays were supported by the Program of Presidium of Russian Academy of Sciences (no. 24). The analytical studies were performed with the equipment of the Center of Collective Use, Lomonosov Moscow State University, with the financial support of the Ministry of Education and Science of the Russian Federation.

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Authors and Affiliations

  1. Research Institute for Genetics and Selection of Industrial Microorganisms, 117545, Moscow, Russia

    T. A. Voeikova, O. A. Zhuravliova, T. S. Gracheva, N. V. Bulushova & V. G. Debabov

  2. Agro-Technological Institute, People’s Friendship University, 117198, Moscow, Russia

    O. A. Zhuravliova

  3. Lomonosov Moscow State University, 119991, Moscow, Russia

    T. T. Ismagulova & K. V. Shaitan

  4. Semenov Institute of Chemical Physics, Russian Academy of Sciences, 119991, Moscow, Russia

    K. V. Shaitan

Authors
  1. T. A. Voeikova
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  2. O. A. Zhuravliova
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  3. T. S. Gracheva
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  4. N. V. Bulushova
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  5. T. T. Ismagulova
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  6. K. V. Shaitan
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  7. V. G. Debabov
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Correspondence to T. A. Voeikova or V. G. Debabov.

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Translated by V. Kudrinskaya

Abbreviations: IR—infrared; CL—culture liquid, OD—optical density, TEM—transmission electron microscopy; LB medium—Luria–Bertani medium; MM—molecular mass standards; UV—ultraviolet; EDS—energy dispersive X-ray spectroscopy; and TSB—Tripton Soya Broth.

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Voeikova, T.A., Zhuravliova, O.A., Gracheva, T.S. et al. Optimization of Microbial Synthesis of Silver Sulfide Nanoparticles. Appl Biochem Microbiol 54, 800–807 (2018). https://doi.org/10.1134/S0003683818080070

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