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Biofilms of nitrile-hydrolyzing bacteria: Dynamics of growth, resistance to toxic substances, and biotechnological potential

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Abstract

Monospecies and mixed bacterial biofilms of Rhodococcus ruber gt1, Pseudomonas fluorescens C2, Alcaligenes faecalis 2, and Rh. erythropolis 11-2 were obtained during growth in presence of the carriers. The transformation of aliphatic and aromatic nitriles by the biofilms of nitrile-hydrolyzing bacteria, as well as the growth dynamics of Rh. ruber gt1 and P. fluorescens C2 biofilms and their resistance to toxic substrates and products of nitrile hydrolysis, were studied. It was shown that the P. fluorescens C2 biofilm mass and total ATP content reached the maxima after 1 day of growth, whereas Rh. ruber gt1 reached them after 3–4 days of cultivation. The biofilms of Rh. ruber gt1 and P. fluorescens C2 were more resistant to the effects of high concentrations of acrylamide and acrylonitrile and had a greater adaptive capacity than planktonic cells.

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Abbreviations

HPLC:

high-performance liquid chromatography

DMSO:

dimethyl sulfoxide

CFU:

colony-forming units

OD:

optical density

LB medium:

Luria–Bertani medium

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Urals Branch, Russian Academy of Sciences, Perm, 614081, Russia

    Yu. G. Maksimova, A. Yu. Maksimov & V. A. Demakov

  2. Perm State National Research University, Perm, 614990, Russia

    Yu. G. Maksimova, A. Yu. Maksimov & V. A. Demakov

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  1. Yu. G. Maksimova
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  2. A. Yu. Maksimov
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  3. V. A. Demakov
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Correspondence to Yu. G. Maksimova.

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Original Russian Text © Yu.G. Maksimova, A.Yu. Maksimov, V.A. Demakov, 2015, published in Biotekhnologiya, 2015, No. 4, pp. 39–51.

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Maksimova, Y.G., Maksimov, A.Y. & Demakov, V.A. Biofilms of nitrile-hydrolyzing bacteria: Dynamics of growth, resistance to toxic substances, and biotechnological potential. Appl Biochem Microbiol 52, 739–749 (2016). https://doi.org/10.1134/S0003683816080068

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  • DOI: https://doi.org/10.1134/S0003683816080068

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