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Methods of microorganism immobilization for dynamic atomic-force studies (review)

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Abstract

Atomic-force microscopy (AFM) is an efficient method for studying the surface ultrastructure and nanomechanical properties of biological objects, including microorganisms. A correctly selected method of microorganism immobilization that provides a strong attachment of cells on the surface of a biologically inert substrate and preservation of their native properties is important for AFM scanning in liquid media. Comparative characteristics of methods of microorganism immobilization applied in dynamic AFM studies are discussed in the review. Technologies of mechanical entrapment and chemical binding of cells to a substrate, as well as protein and immunospecific adsorption, are considered.

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Science, Perm, 614081, Russia

    M. S. Kuyukina, I. O. Korshunova, E. V. Rubtsova & I. B. Ivshina

  2. Perm State National Research University, Perm, 614990, Russia

    M. S. Kuyukina, E. V. Rubtsova & I. B. Ivshina

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  1. M. S. Kuyukina
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  2. I. O. Korshunova
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  3. E. V. Rubtsova
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Original Russian Text © M.S. Kuyukina, I.O. Korshunova, E.V. Rubtsova, I.B. Ivshina, 2014, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 1, pp. 7–16.

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Kuyukina, M.S., Korshunova, I.O., Rubtsova, E.V. et al. Methods of microorganism immobilization for dynamic atomic-force studies (review). Appl Biochem Microbiol 50, 1–9 (2014). https://doi.org/10.1134/S0003683814010086

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