Skip to main content
SpringerLink
Log in

Purification and cloning of nicosulfuron-degrading enzymes from Bacillus subtilis YB1

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

The nicosulfuron-degrading enzymes from Bacillus subtilis strain YB1 were purified and their genes were cloned. The proteins of bacterial culture filtrate were precipitated with ammonium sulfate or acetone. The extracellular proteins concentrated by acetone were purified from DEAE-Sepharose Fast Flow chromatography. The four protein peaks eluted from DEAE-column were separated and purified by native PAGE. Three components (P1-1, P3-2, P4-3) had nicosulfuron-degrading activity, and component P4-3 degradated 57.5% of this compound. The molecular weights of the components were 33.5, 54.8 and 37.0 kDa, respectively. The amino acid sequences of nicosulfuron-degrading enzymes from B. subtilis YB1 were determined by MALDI-TOF-MS, indicating these enzymes as manganese ABC transporter, vegetative catalase 1 and acetoin dehydrogenase E1, respectively. Using PCR amplification, genes 918, 1428, 1026 bp in size were detected for the enzymes studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Arias-Estévez, M., López-Periagoa, E., Martínez-Carballob, E., Simal-Gándarab, J., Mejutoc, J.C., and García-Ríod, L., Agr. Ecosyst Environ., 2008, vol. 123, no. 4, pp. 247–260.

    Article  Google Scholar 

  2. Koskinen, W. and Regitano, J., J. Agr. Food Chem., 2008, vol. 56, no. 14, pp. 5801–5805.

    Article  Google Scholar 

  3. Nélieu, S., Perreau, F., Guichon, R., Seguin, F., Bry, C., and Einhorn, J., Anal. Bioanal. Chem., 2005, vol. 382, no. 1, pp. 108–114.

    Article  PubMed  Google Scholar 

  4. Zhang, H., Zhang, X., Mu, W., Wang, J., Pan, H., and Li, Y., J. Environ. Sci. Health B., 2010, vol. 45, no. 6, pp. 501–507.

    Article  PubMed  Google Scholar 

  5. Ma, J.P., Wang, Z., Lu, P., Wang, H., Ali, S., Li, S., and Huang, X., FEMS Microbiol. Lett., 2009, vol. 296, no. 2, pp. 203–209.

    Article  CAS  PubMed  Google Scholar 

  6. Gaultier, J., Farenhorst, A., Cathcart, J., and Goddard, T., Soil. Biol. Biochem., 2008, vol. 40, no. 1, pp. 217–227.

    Article  CAS  Google Scholar 

  7. Luo, W., Zhao, Y.H., Ding, H.T., Lin, X.Y., and Zheng, H.B., J. Hazard. Mater., 2008, vol. 158, no. 1, pp. 208–214.

    Article  CAS  PubMed  Google Scholar 

  8. Zablotowicz, R.M., Weave, M.A., and Locke, M.A., Weed Sci., 2006, vol. 54, pp. 538–547.

    Article  CAS  Google Scholar 

  9. Phillips, T.M., Seech, A.G., Lee, H., and Trevors, J.T., Biodegradation, 2005, vol. 16, no. 4, pp. 363–392.

    Article  CAS  PubMed  Google Scholar 

  10. Yu, Y.L., Wang, X., Luo, M.Y., Yang, J.F., Yu, J.Q., and Fan, D.F., Chemosphere, 2005, vol. 60, no. 4, pp. 460–466.

    Article  CAS  PubMed  Google Scholar 

  11. Gu, J.G., Fan, Y.Z., and Gu, J.D., Chemosphere, 2003, vol. 52, no. 9, pp. 1515–1521.

    Article  CAS  PubMed  Google Scholar 

  12. Gu, L.F., Jiang, J.D., Li, X.H., Ali, S.W., and Li, S.P., Curr. Microbiol., 2007, vol. 55, no. 5, pp. 420–426.

    Article  CAS  Google Scholar 

  13. Xu, J., Li, X.S., Xu, Y.J., Qiu, L.H., and Pan, C.P., Chemosphere, 2009, vol. 74, no. 5, pp. 682–687.

    Article  CAS  PubMed  Google Scholar 

  14. Cai, T., Qian, L.H., Cai, S., and Chen, L.W., Curr. Microbiol., 2011, vol. 62, no. 2, pp. 570–577.

    Article  CAS  PubMed  Google Scholar 

  15. Wackett, L., Curr. Opin. Microbiol., 2009, vol. 12, no. 3, pp. 244–251.

    Article  CAS  PubMed  Google Scholar 

  16. Huang, X., He, J., Sun, J.Q., Pan, J.J., Sun, X.F., and Li, S.P., Int. Biodeter. Biodegr., 2007, vol. 60, no. 3, pp. 152–158.

    Article  CAS  Google Scholar 

  17. Liu, Y.H., Chung, Y.C., and Xiong, Y., Appl. Environ. Microbiol., 2001, vol. 67, no. 8, pp. 3764–3749.

    Article  Google Scholar 

  18. Pizzul, L., Castillo, M., and Stenstr, J., Biodegradation, 2009, vol. 20, no. 6, pp. 751–759.

    Article  CAS  PubMed  Google Scholar 

  19. Tomasek, P.H. and Karns, J.S., J. Bacteriol., 1989, vol. 171, no. 7, pp. 4038–4044.

    CAS  PubMed Central  PubMed  Google Scholar 

  20. Cui, Z.L., Li, S.P., and Fu, G.P., Appl. Environ. Microbiol., 2001, vol. 67, no. 10, pp. 4922–4925.

    Article  CAS  Google Scholar 

  21. Li, X.H., He, J., and Li, S.P., Res. Microbiol., 2007, vol. 158, no. 2, pp. 143–149.

    Article  CAS  PubMed  Google Scholar 

  22. Yang, Y.J, Liu, S., Wu, L.F., Pang, M.H., Tao, B., and Zhang, J.L., Chinese J. Pesticide Sci., 2007, vol. 9, no. 3, pp. 275–279.

    CAS  Google Scholar 

  23. Reisfeld, R.A., Lewis, U.J., and Williams, D.E., Nature, 1962, vol. 195, pp. 281–283.

    Article  CAS  PubMed  Google Scholar 

  24. LaemmLi, U.K., Nature, 1970, vol. 227, pp. 680–685.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Plant Protection, Agricultural University of Hebei, Baoding, 071001, Hebei Province, China

    Z. H. Kang, C. C. Ren, J. L. Zhang, X. Li & X. J. Wei

  2. College of Life Sciences, Agricultural University of Hebei, Baoding, 071001, Hebei Province, China

    J. G. Dong

Authors
  1. Z. H. Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. C. Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. L. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. G. Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. X. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. X. J. Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. L. Zhang.

Additional information

Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2014, Vol. 50, No. 1, pp. 39–43.

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kang, Z.H., Ren, C.C., Zhang, J.L. et al. Purification and cloning of nicosulfuron-degrading enzymes from Bacillus subtilis YB1. Appl Biochem Microbiol 50, 30–34 (2014). https://doi.org/10.1134/S0003683814010049

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683814010049

Keywords

Search

Navigation