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Degradation of glyphosate and other pesticides by ligninolytic enzymes

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Abstract

The ability of pure manganese peroxidase (MnP), laccase, lignin peroxidase (LiP) and horseradish peroxidase (HRP) to degrade the widely used herbicide glyphosate and other pesticides was studied in separate in vitro assays with addition of different mediators. Complete degradation of glyphosate was obtained with MnP, MnSO4 and Tween 80, with or without H2O2. In the presence of MnSO4, with or without H2O2, MnP also transformed the herbicide, but to a lower rate. Laccase degraded glyphosate in the presence of (a) 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), (b) MnSO4 and Tween 80 and (c) ABTS, MnSO4 and Tween 80. The metabolite AMPA was detected in all cases where degradation of glyphosate occurred and was not degraded. The LiP was tested alone or with MnSO4, Tween 80, veratryl alcohol or H2O2 and in the HRP assay the enzyme was added alone or with H2O2 in the reaction mixture. However, these enzymes did not degrade glyphosate. Further experiments using MnP together with MnSO4 and Tween 80 showed that the enzyme was also able to degrade glyphosate in its commercial formulation Roundup® Bio. The same enzyme mixture was tested for degradation of 22 other pesticides and degradation products present in a mixture and all the compounds were transformed, with degradation percentages ranging between 20 and 100%. Our results highlight the potential of ligninolytic enzymes to degrade pesticides. Moreover, they suggest that the formation of AMPA, the main metabolite of glyphosate degradation found in soils, can be a result of the activity of lignin-degrading enzymes.

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Acknowledgments

The authors thank Elisabet Börjesson (Department of Microbiology, SLU) for her technical help in the analyses of glyphosate and AMPA. This project was financed by the Centre for Chemical Pesticides (CKB) at SLU and by the Foundation for Strategic Environmental Research (MISTRA) by funding the research programme Domestication of Microorganisms (DOM).

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Authors and Affiliations

  1. Department of Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7025, 750 07, Uppsala, Sweden

    Leticia Pizzul & John Stenström

  2. JTI, Swedish Institute of Agricultural and Environmental Engineering, P.O. Box 7033, 750 07, Uppsala, Sweden

    María del Pilar Castillo

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  1. Leticia Pizzul
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  2. María del Pilar Castillo
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Correspondence to Leticia Pizzul.

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Pizzul, L., Castillo, M.d.P. & Stenström, J. Degradation of glyphosate and other pesticides by ligninolytic enzymes. Biodegradation 20, 751–759 (2009). https://doi.org/10.1007/s10532-009-9263-1

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