Abstract
The dependence of secretion efficiency in Pichia pastoris cells on the copy number of proregions in leader polypeptides has been studied. The humanized light kappa-chain of the murine H3-1 antibody was used as a reporter protein. The leader pre-pro-polypeptides were composed of the signal peptide (preregion) from the α-factor precursors of Saccharomyces cerevisiae and a variable number of proregions from the prepro-precursors of the α-factor or the Hsp150p protein of S. cerevisiae or Hsp150p of P. pastoris. An increase in the proregion copy number either resulted in an almost 1.5-fold increase or a fivefold decrease in secretion depending on the proregion used. It was concluded that the enhancement of the proregion copy number could be of potential value for the intensification of protein secretion in P. pastoris.
Similar content being viewed by others
References
Kohara, A., Yamamoti, Y., and Kikuchi, M., Biosci. Biotechnol. Biochem., 1994, vol. 58, pp. 779–781.
Yurimoto, H., Yamane, M., Kikuchi, Y., Matsui, H., Kato, N., and Sakai, Y., Biosci. Biotechnol. Biochem., 2004, vol. 68, pp. 2058–2069.
Tanaka, H., Okuno, T., Moriyama, S., Muguruma, M., and Ohta, K., J. Biosci. Bioeng., 2004, vol. 98, pp. 338–343.
Hahm, M.S. and Chung, B.H., J. Biotechnol. Bioproc. Eng., 2001, vol. 6, pp. 306–309.
Chaudhuri, B., Steube, K., and Syephan, C., Eur. J. Biochem., 1992, vol. 206, pp. 793–800.
Takahashi, S., Ueda, M., and Tanaka, A., Appl. Microbiol. Biotechnol., 2001, vol. 55, pp. 454–462.
Kozlov, D.G. and Yagudin, T.A., Biotechnol. Lett., 2008, vol. 30, pp. 1661–1663.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.V. Tyurin, I.I. Gubaydullin, S.E. Cheperegin, B.D. Efremov, D.G. Kozlov, 2012, published in Biotekhnologiya, 2012, No. 3, pp. 48–52.
Rights and permissions
About this article
Cite this article
Tyurin, O.V., Gubaydullin, I.I., Cheperegin, S.E. et al. Amplification of leader proregions as a mean to increase the secretion of antibody fragments in the Pichia pastoris yeast. Appl Biochem Microbiol 49, 656–659 (2013). https://doi.org/10.1134/S0003683813070065
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683813070065