Abstract
We have studied the efficiency of N-terminal processing of the antibody light chain depending on the structure of the leader sequence when expressed in the yeast Pichia pastoris. The humanized light kappa-chain of the murine antibody H3-1 and the Saccharomyces cerevisiae α-factor pre-pro-leader sequence (pre-pro-α-F) were used as models. The use of pre-region of the pre-pro-α-F alone or together with the Glu-Ala-linker leads to the slightly increased yield of the secreted L-chain but was accompanied by the incomplete N-terminal processing of the secreted product.
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This work was partially supported by the Russian Federal Agency for Science and Innovation, State Contract No. 02.531.11.9003.
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Kozlov, D.G., Yagudin, T.A. Antibody fragments may be incorrectly processed in the yeast Pichia pastoris . Biotechnol Lett 30, 1661–1663 (2008). https://doi.org/10.1007/s10529-008-9739-9
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DOI: https://doi.org/10.1007/s10529-008-9739-9