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Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from waste products of a salt-mining plant

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Abstract

Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from technogenic mineral waste products of the BKRU1 “Uralkalii” plant was investigated (Berezniki, Perm krai). The R. wratislaviensis KT112-7 was shown to utilize increased concentrations of o-phthalic (o-PA) (8 g/L) and benzoic (BA) (3.4 g/L) acids. The strain grows with o-PA, BA, and biphenyl at a NaCl content of up to 75, 90 and 100 g/L in the culture medium, respectively. Based on an analysis of the metabolic profile and nucleotide sequences of the bphA1, benA, and phtB genes, the strain KT112-7 was found to degrade o-PA via the formation of 3,4-dihydroxyphthalic and 3,4-dihydroxybenzoic acids. Degradation of biphenyl proceeds via the formation of BA and then at low concentrations of NaCl (up to 50 g/L) via the formation of 4-hydroxybenzoic acid with its subsequent oxidation, while at high concentrations of NaCl (over 60 g/L)-via the direct oxidation of benzoic acid to resultant catechol. These data indicate that the Rhodococcus wratislaviensis KT112-7 is a promising strain in the development of new biotechnologies directed for utilization (transformation) of aromatic compounds, including the conditions of increased mineralization.

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References

  1. http://www.atsdr.cdc.gov/toxprofiles/tp17.html

  2. Bachurin, B.A. and Odintsova, G.A., Gorn. Inform.-Analit. Byull., 2009, no. 7, pp. 374–380.

    Google Scholar 

  3. Le Borgne, S., Paniagua, D., and Vazquez-Duhalt, R., J. Mol. Microbiol. Biotechnol., 2008, vol. 15, nos 2–3, pp. 74–92.

    Article  PubMed  Google Scholar 

  4. Plotnikova, E.G., Rybkina, D.O., Anan’ina, L.N., Yastrebova, O.V., and Demakov, V.A., Russ. J. Ecol., 2006, vol. 36, no. 4, pp. 261–268.

    Google Scholar 

  5. Anan’ina, L.N., Yastrebova, O.V., Demakov, V.A., and Plotnikova, E.G., Antonie van Leeuwenhoek, 2011, vol. 100, no. 2, pp. 309–316.

    Article  PubMed  Google Scholar 

  6. de Carvalho, C.C.C.R., Res. Microbiol., 2012, vol. 163, no. 2, pp. 125–136.

    Article  PubMed  Google Scholar 

  7. Solyanikova, I.P., Travkin, V.M., Rybkina, D.O., Plotnikova, E.G., and Golovleva, L.A., J. Environ. Sci. Health, 2008, vol. 43, no. 3, pp. 241–252.

    Article  CAS  Google Scholar 

  8. Raymond, R.L., Dev. Ind. Microbiol., 1961, vol. 2, no. 1, pp. 23–32.

    CAS  Google Scholar 

  9. Versalovic, J., Schneider, M., de Bruijn, F.J., and Lupski, J.R., Methods Cell. Mol. Biol., 1994, vol. 5, no. 1, pp. 25–40.

    CAS  Google Scholar 

  10. Manual of Methods for General Bacteriology, Gerhardt, P., Gerhardt, R., Murray, R., Costilou, R., Nester, J., Wood, W., Krieg, N., and Phillips, G., Eds., Washington: Amer. Soc. Microbiol., 1981. Translated under the title Metody obshchei bakteriologii, Moscow: Mir, 1983, vol. 1, pp. 16–89, 277–511; 1984, vol. 2, pp. 283–373; 1984, vol. 3, pp. 8–97.

    Google Scholar 

  11. Tiirola, M.A., Mannisto, M.K., Puhakka, J.A., and Kulomaa, M.S., Appl. Environ. Microbiol., 2002, vol. 68, no. 1, pp. 173–180.

    Article  PubMed  CAS  Google Scholar 

  12. Thompson, J.D., Higgins, D.G., and Gibson, T.J., Nucleic Acids Res., 1994, vol. 22, no. 22, pp. 4673–4680.

    Article  PubMed  CAS  Google Scholar 

  13. Egorova, D.O., Demakov, V.A., and Plotnikova, E.G., Appl. Biochem. Microbiol., 2010, vol. 46, no. 6, pp. 592–598.

    Article  CAS  Google Scholar 

  14. Eaton, R.W., J. Bacteriol., 2001, vol. 183, no. 12, pp. 3689–3703.

    Article  PubMed  CAS  Google Scholar 

  15. Paniker, G., Mojib, N., Aislabie, J., and Bej, A.K., Antonie van Leeuwenhoek, 2010, vol. 97, no. 3, pp. 275–287.

    Article  Google Scholar 

  16. Baldwin, B.R., Nakatsu, C.H., and Nies, L., Appl. Environ. Microbiol., 2003, vol. 69, no. 6, pp. 3350–3358.

    Article  PubMed  CAS  Google Scholar 

  17. Mesarch, M.B., Nakatsu, C.H., and Nies, L., Appl. Environ. Microbiol., 2000, vol. 66, no. 2, pp. 678–683.

    Article  PubMed  CAS  Google Scholar 

  18. Baggi, G., Bernasconi, S., Zangarossi, M., Cavalca, L., and Andereoni, V., Int. Biodeter. Biodegrad., 2008, vol. 62, no. 1, pp. 57–64.

    Article  CAS  Google Scholar 

  19. Jouanneau, Y. and Meyer, C., Appl. Environ. Microbiol., 2006, vol. 72, no. 7, pp. 4726–4734.

    Article  PubMed  CAS  Google Scholar 

  20. Xu, S., Ju, J., Misono, H., and Ohnishi, K., Gene, 2006, vol. 368, no. 1, pp. 126–137.

    Article  PubMed  CAS  Google Scholar 

  21. Hong, Q., Dong, X., He, L., Jiang, X., and Li, S., Int. Biodeter. Biodegrad., 2009, vol. 63, no. 4, pp. 365–370.

    Article  CAS  Google Scholar 

  22. Rodrigues, J.L.M., Kachel, C.A., Aiello, M.R., Qensen, J.F., Maltseva, O.V., Tsoi, T.V., and Tiedje, J.M., Appl. Environ. Microbiol., 2006, vol. 72, no. 4, pp. 2476–2482.

    Article  PubMed  CAS  Google Scholar 

  23. Garcia, M.T., Ventosa, A., and Mellado, E., FEMS Microbiol. Ecol., 2005, vol. 54, no. 1, pp. 97–109.

    Article  PubMed  CAS  Google Scholar 

  24. Stingley, R.L., Brezna, B., Khan, A.A., and Cerniglia, C.E., Microbiology, 2004, vol. 150, no. 11, pp. 3749–3761.

    Article  PubMed  CAS  Google Scholar 

  25. Vedler, E., in Megaplasmids and the Degradation of Aromatic Compounds by Soil Bacteria. Microbial Megaplasmids, Schwartz, E., Ed., Berlin, Heidelberg: Springer-Verlag, 2009, pp. 33–53.

  26. Murad, S., Hasan, F., Shah, A.A., Hameed, A., and Ahmed, S., Pakist. J. Bot., 2007, vol. 39, no. 5, pp. 1833–1841.

    Google Scholar 

  27. Vamsee-Krishna, C., Mohan, Y., and Phale, P.S., Appl. Microbiol. Biotechnol., 2006, vol. 72, no. 6, pp. 1263–1269.

    Article  PubMed  CAS  Google Scholar 

  28. Oie, C.S.I., Albaugh, C.E., and Peyton, B.M., Water Res., 2007, vol. 41, no. 6, pp. 1235–1242.

    Article  PubMed  CAS  Google Scholar 

  29. Xu, X.-R., Li, H.-B., and Gu, J.-D., Ecotoxicol. Environ. Safety, 2007, vol. 68, no. 3, pp. 379–385.

    Article  PubMed  CAS  Google Scholar 

  30. Li, A., Qu, Y., Zhou, J., and Gou, M., J. Environ. Sci., 2009, vol. 21, no. 2, pp. 211–217.

    Article  CAS  Google Scholar 

  31. Pieper, D.H., Appl. Microbiol. Biotechnol., 2005, vol. 67, no. 2, pp. 170–191.

    Article  PubMed  CAS  Google Scholar 

  32. Reddy, C.C. and Vaidyanathan, C.S., Arch. Biochem. Biophys., 1976, vol. 177, no. 2, pp. 488–498.

    Article  PubMed  CAS  Google Scholar 

  33. Xie, N., Tang, H., Feng, J., Tao, F., Ma, C., and Xu, P., Biochem. Eng. J., 2009, vol. 46, no. 1, pp. 79–82.

    Article  CAS  Google Scholar 

  34. van Duuren, J.B.J.H., Wijte, D., Leprince, A., Karge, B., Puchalka, J., Wery, J., and Santos, V.A.P., M., Eggink G., Mars A.E, J. Bacteriol., 2011, vol. 156, no. 3, pp. 163–172.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, Russia

    D. O. Egorova, E. S. Korsakova, V. A. Demakov & E. G. Plotnikova

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  1. D. O. Egorova
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  2. E. S. Korsakova
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  3. V. A. Demakov
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  4. E. G. Plotnikova
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Correspondence to D. O. Egorova.

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Original Russian Text © D.O. Egorova, E.S. Korsakova, V.A. Demakov, E.G. Plotnikova, 2013, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2013, Vol. 49, No. 3, pp. 267–278.

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Egorova, D.O., Korsakova, E.S., Demakov, V.A. et al. Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from waste products of a salt-mining plant. Appl Biochem Microbiol 49, 244–255 (2013). https://doi.org/10.1134/S0003683813030071

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  • DOI: https://doi.org/10.1134/S0003683813030071

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