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Effect of submerged cultivation conditions and inducers on biosynthesis of extracellular laccase by a Trametes versicolor 1666 strain

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Abstract

Genetic analysis of basidiomycete Trametes versicolor 1666 from the Komarov Botanical Institute (LEBIN) Collection has been carried out that verified the phylogenetic position of the strain and revealed the presence of a single laccase gene. A study of cultivation dynamics of basidiomycete showed a low level of laccase, peroxidase, and Mn-peroxidase production on glucose-peptone and mineral media. The level of laccase activity in the culture liquid (CL) remained practically unchanged during cultivation in a medium optimized using full factorial experiment as compared to the standard medium. An addition of laccase inducers (syringaldazine, caffeic acid, and synaptic acid) had no effect on the enzyme activity in the culture liquid. Real time PCR showed a lack of reliable difference in the level of laccase gene expression when cultivating the strain under optimal conditions with and without copper ions in the medium. The transcription behavior of laccase gene and occurrence of enzyme activity when cultivating T. versicolor 1666 strain testify to the enzyme constitutive synthesis and the existence of additional regulatory ways in the laccase gene expression.

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Abbreviations

DIC:

differential interference contrast

CL:

culture liquid

OD:

optical density

PCR:

polymerase chain reaction

RT PCR:

real time PCR

FF:

full factorial experiment

EDTA:

ethylenediaminetetraacetic acid

ABTS:

2,2′-azinobis(3-ethylbenzothiazoline-6-sulphonic acid)

dNTP:

deoxyribonucleotide triphosphate

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Authors and Affiliations

  1. Komarov Botanical Institute, Russian Academy of Sciences, St. Petersburg, 197376, Russia

    N. V. Shakhova & N. V. Psurtseva

  2. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071, Russia

    S. A. Golenkina, E. V. Stepanova, D. S. Loginov, T. V. Fedorova & O. V. Koroleva

Authors
  1. N. V. Shakhova
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  2. S. A. Golenkina
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  3. E. V. Stepanova
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  4. D. S. Loginov
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  5. N. V. Psurtseva
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  6. T. V. Fedorova
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  7. O. V. Koroleva
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Correspondence to O. V. Koroleva.

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Original Russian Text © N.V. Shakhova, S.A. Golenkina, E.V. Stepanova, D.S. Loginov, N.V. Psurtseva, T.V. Fedorova, O.V. Koroleva, 2011, published in Biotekhnologiya, 2011, No. 2, pp. 34–44.

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Shakhova, N.V., Golenkina, S.A., Stepanova, E.V. et al. Effect of submerged cultivation conditions and inducers on biosynthesis of extracellular laccase by a Trametes versicolor 1666 strain. Appl Biochem Microbiol 47, 808–816 (2011). https://doi.org/10.1134/S0003683811090055

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  • DOI: https://doi.org/10.1134/S0003683811090055

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