Skip to main content
SpringerLink
Log in

Cloning and sequence analysis of complete gene encoding an alkaline lipase from Penicillium cyclopium

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

The complete gene (PG37 lip) encoding an alkaline lipase (PG37 LipI) was cloned from the genomic DNA of Penicillium cyclopium PG37. The cloned PG37 lipI is 2.020 bp in length, consisting of 632 bp of the 5′ flanking promoter region and 1.388 bp of the downstream fragment that contains 6 exons and 5 short introns. The promoter region harbors putative TATA box, CAAT box and several transcription factor binding sites. The open reading frame (ORF) encodes a PG37 LipI of 285 amino acid residues, which was predicted to contain a 20-aa signal peptide, a 7-aa propeptide and a 258-aa mature peptide with a conserved motif Gly-X-Ser-X-Gly. However, PG37 Lip shows only 32%, 30%, 28% and 26% identity with lipases of Aspergillus parasiticus, Penicillium camembertii, Thermomyces lanuginosus and Rhizomucor miehei, respectively. It was predicted that the main secondary structures of PG37 Lip are α-helix and random coil. Three amino acid residues, Ser132-Asp188-His241, compose the enzymatic active center in the tertiary structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Villeneuve, P., Muderhwa, J.M., Graille, J., and Haas, M.J., J. Mol. Catal. B: Enzym., 2000, vol. 9, pp. 113–148.

    Article  CAS  Google Scholar 

  2. Sun, S.Y., Xu, Y., and Wang, D., Bioresour. Technol., 2009, vol. 100, pp. 2607–2612.

    Article  PubMed  CAS  Google Scholar 

  3. Hasan, F., Shah, A.A., and Hameed, A., Enzyme Microb. Technol., 2006, vol. 39, pp. 235–251.

    Article  CAS  Google Scholar 

  4. Saxena, R.K., Sheoran, A., Giri, B., and Davidson, W.S., J. Microbiol. Methods, 2003, vol. 52, pp. 1–18.

    Article  PubMed  CAS  Google Scholar 

  5. Korneeva, O.S., Popova, T.N., Kapranchikov, V.S., and Motina, E.A., Appl. Biochem. Microbiol., 2008, vol. 45, pp. 349–355.

    Article  Google Scholar 

  6. Beer, H.D., McCarthy, J.E.G., Bornscheuer, U.T., and Schmid, R.D., Biochim. Biophys. Acta, 1998, vol. 1399, pp. 173–180.

    PubMed  CAS  Google Scholar 

  7. Hube, B., Stehr, F., Bossenz, M., Mazur, A., Kretschmar, M., and Schafer, W., Arch. Microbiol., 2000, vol. 174, pp. 362–374.

    Article  PubMed  CAS  Google Scholar 

  8. Eggert, T., Van Pouderoyen, G., Pencreach, G., Douchet, I., Verger, R., Dijkstra, B.W., and Jaeger, K.E., Colloid Surf. B: Biointerface, 2002, vol. 26, pp. 37–46.

    Article  CAS  Google Scholar 

  9. Ollis, D.L., Cheah, E., Cygler, M., Dijkstra, B., Frolow, F., Franken, S.M., Harel, M., Remington, S.J., Silman, I., Schrag, J., Sussman, J.L., Verschueren, K.H.G., and Goldman, A., Protein Eng., 1992, vol. 5, pp. 197–211.

    Article  PubMed  CAS  Google Scholar 

  10. Brady, L., Brzozowski, A.M., Derewenda, Z.S., Dodson, E., Dodson, G.G., Tolley, S., Turkenburg, J.P., Christiansen, L., Huge-Jensen, B., Norskov, L., Thim, L., and Menge, U., Nature, 1990, vol. 343, pp. 767–770.

    Article  PubMed  CAS  Google Scholar 

  11. Zhang, B., Yang, J.K., and Yan, Y.J., Biotechnol. Bulletin, 2007, vol. 2, pp. 52–57.

    Google Scholar 

  12. Sharma, R., Soni, S.K., Vohra, R.M., Gupta, L.K., and Gupta, J.K., Process Biochem., 2002, vol. 37, pp. 1075–1084.

    Article  CAS  Google Scholar 

  13. Niu, W.N., Li, Z.P., Zhang, D.W., Yu, M.R., and Tan, T.W., J. Mol. Catal. B: Enzym., 2006, vol. 43, pp. 33–39.

    Article  CAS  Google Scholar 

  14. Cox, M., Gerritse, G., Dankmeyer, L., and Quax, W.J., J. Biotechnol., 2001, vol. 86, pp. 9–17.

    Article  PubMed  CAS  Google Scholar 

  15. Wu, M.C., Qian, Z.K., Jiang, P.H., Min, T.S., Sun, C.R., and Huang, W.D., Lipids, 2003, vol. 38, pp. 191–199.

    Article  PubMed  CAS  Google Scholar 

  16. Sambrook, J. and Russell, D.W., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor, 2001.

    Google Scholar 

  17. Wendland, J., Lengeler, K.B., and Kothe, E., Fungal Genet. Newslett., 1996, vol. 43, pp. 54–55.

    Google Scholar 

  18. Reed, D.S. and Gibson, J.B., Biochem. Genet., 1994, vol. 32, pp. 161–179.

    Article  PubMed  CAS  Google Scholar 

  19. Lampidonis, A.D., Stravopodis, D.J., Voutsinas, G.E., Messini-Nikolaki, N., Stefos, G.C., Margaritis, L.H., Argyrokastritis, A., Bizelis, I., and Rogdakis, E., Gene, 2008, vol. 427, pp. 65–79.

    Article  PubMed  CAS  Google Scholar 

  20. Wenkel, S., Turck, F., Singer, K., Gissot, L., Le Gourrierec, J., Samach, A., and Coupland, G., Plant Cell, 2006, vol. 18, pp. 2971–2984.

    Article  PubMed  CAS  Google Scholar 

  21. Sayari, A., Frikha, F., Miled, N., Mtibaa, H., Ben Ali Y., Verger, R., and Gargouri, Y., FEBS Lett., 2005, vol. 579, pp. 976–982.

    Article  PubMed  CAS  Google Scholar 

  22. Nagao, T., Shimada, Y., Sugihara, A., and Tominaga, Y., J. Ferment. Bioeng., 1996, vol. 81, pp. 488–492.

    Article  CAS  Google Scholar 

  23. Ishida, Y. and Leal, W.S., Insect Biochem. Mol. Biol., 2002, vol. 32, pp. 1775–1780.

    Article  PubMed  CAS  Google Scholar 

  24. Tyndall, J.D.A., Sinchaikul, S., Fothergill-Gilmore, L.A., Taylor, P., and Walkinshaw, M.D., J. Mol. Biol., 2002, vol. 323, pp. 859–869.

    Article  PubMed  CAS  Google Scholar 

  25. Karkhane, A.A., Yakhchali, B., Jazii, F.R., and Bambai, B., J. Mol. Catal. B-Enzym., 2009, vol. 61, pp. 162–167.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, PR China

    H. M. Zhang

  2. School of Medicine and Pharmaceutics, Jiangnan University, Wuxi, Jiangsu, 214122, PR China

    M. C. Wu, J. Guo & J. F. Li

Authors
  1. H. M. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. C. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. F. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. M. Zhang.

Additional information

Published in Russian in Prikladnaya Biokhimiya i Mikrobiologiya, 2011, Vol. 47, No. 6, pp. 642–649.

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, H.M., Wu, M.C., Guo, J. et al. Cloning and sequence analysis of complete gene encoding an alkaline lipase from Penicillium cyclopium . Appl Biochem Microbiol 47, 586–593 (2011). https://doi.org/10.1134/S0003683811060135

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0003683811060135

Keywords

Search

Navigation