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Piezoquartz immunosensors for assessing the interactions between Yersinia enterocolitica lipopolysaccharides and antibodies to them

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Abstract

The utility of a piezoquartz immunosensor coated with lipopolysaccharides (LPS) for the quantification of antibody specificities was demonstrated. Immunochemical reactions were monitored according to the changes in the weight of sensor bioreceptor layer with high sensitivity (detection limit, 1.3 μg/ml) and assay rate (10 min) without any additional labels. The capabilities of this sensor were demonstrated by the example of quantifying the cross-reactivity of blood serum antibodies with the LPS of Yersinia enterocolitica serotypes O:3, O:5, O:5.27, O:6.30, and O:6.31. The proposed approach is promising for clinical diagnostics of yersiniosis, an infectious intestinal disease.

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Authors and Affiliations

  1. Lipetsk State Technical University, Lipetsk, 398600, Russia

    E. N. Kalmykova, E. S. Dergunova & T. N. Ermolaeva

  2. Pacific Institute of Bioorganic Chemistry, Far Eastern Division, Russian Academy of Sciences, Vladivostok, 690022, Russia

    R. P. Gorshkova & N. A. Komandrova

Authors
  1. E. N. Kalmykova
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  2. E. S. Dergunova
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  3. T. N. Ermolaeva
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  4. R. P. Gorshkova
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  5. N. A. Komandrova
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Correspondence to E. N. Kalmykova.

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Original Russian Text © E.N. Kalmykova, E.S. Dergunova, T.N. Ermolaeva, R.P. Gorshkova, N.A. Komandrova, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 45, No. 4, pp. 410–416.

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Kalmykova, E.N., Dergunova, E.S., Ermolaeva, T.N. et al. Piezoquartz immunosensors for assessing the interactions between Yersinia enterocolitica lipopolysaccharides and antibodies to them. Appl Biochem Microbiol 44, 372–377 (2008). https://doi.org/10.1134/S0003683808040066

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  • DOI: https://doi.org/10.1134/S0003683808040066

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