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Optimization of the protocol for constructing transgenic plants of the cabbage Brassica oleracea var. capitata

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Abstract

The strain Agrobacterium tumefaciens GV3101, which contains the pBar vector carrying the phosphinothricin acetyltransferase gene (bar) under the control of the 35SCMoV promoter and NOS 3′ terminator, was used for genetic transformation of four cabbage lines, Ges-3, Drv-2, Zmu 7, and Meg 2. The effect of different concentrations and combinations of phytohormones was studied, which allowed for choosing the cultivation conditions that provided a 63–87% regeneration efficiency. It was demonstrated that concerted action by natural and synthetic cytokinins is necessary for the lines studied. Overall, 26 transgenic plants were obtained using the optimized protocol for agrobacterial transformation. The transgenic nature of these plants was confirmed by PCR and dot-blot hybridization.

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Authors and Affiliations

  1. Bioengineering Center, Russian Academy of Sciences, Moscow, 117312, Russia

    T. N. Gribova, A. M. Kamionskaya & K. G. Skryabin

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  1. T. N. Gribova
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  2. A. M. Kamionskaya
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  3. K. G. Skryabin
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Original Russian Text © T.N. Gribova, A.M. Kamionskaya, K.G. Skryabin, 2006, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2006, Vol. 42, No. 5, pp. 593–598.

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Gribova, T.N., Kamionskaya, A.M. & Skryabin, K.G. Optimization of the protocol for constructing transgenic plants of the cabbage Brassica oleracea var. capitata . Appl Biochem Microbiol 42, 519–524 (2006). https://doi.org/10.1134/S0003683806050139

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  • DOI: https://doi.org/10.1134/S0003683806050139

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