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Spectroscopic Techniques for Estimation of Physiological State of Blue-Green Algae after Weak External Action

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Oceanology Aims and scope

Abstract—

Nowadays cyanobacterial blooms in open reservoirs and estuaries became one of the most important ecological problem. The optimal way to solve this problem is to develop innovative methods for controlling the number of bloom-forming cyanobacteria based on weak external actions, which have no serious consequences for the entire ecological system. A novel efficient technique for in vivo estimation of cyanobacterial viability for online ecological monitoring of the results of weak external actions was elaborated by using a combination of different spectroscopic methods. It has been shown that the results obtained by means of conventional spectrophotometry and fluorimetry for cyanobacterial culture as a whole and the data obtained by fluorescent microscopic spectroscopy applied to a single cell are strictly related.

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ACKNOWLEDGMENTS

All studies were carried out using the equipment of the Resource Center “Development of Molecular and Cell Technologies” of the Research Park of St. Petersburg State University and the “Chromas” Center. The authors individually thank the Resource Center core facility Center for Culture Collection of Microorganisms of the Research Park of St. Petersburg State University for providing samples of cyanobacteria strains.

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Authors and Affiliations

  1. St. Petersburg Electrotechnical University LETI, 197376, St. Petersburg, Russia

    N. Yu. Grigoryeva & A. A. Liss

  2. St. Petersburg State University, 199034, St. Petersburg, Russia

    L. V. Chistyakova

Authors
  1. N. Yu. Grigoryeva
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  2. L. V. Chistyakova
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  3. A. A. Liss
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Correspondence to N. Yu. Grigoryeva.

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Translated by G. Karabashev

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Grigoryeva, N.Y., Chistyakova, L.V. & Liss, A.A. Spectroscopic Techniques for Estimation of Physiological State of Blue-Green Algae after Weak External Action. Oceanology 58, 923–931 (2018). https://doi.org/10.1134/S0001437018060061

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  • DOI: https://doi.org/10.1134/S0001437018060061

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