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DNA aptamer-based sorbents for binding human IgE

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Abstract

DNA aptamer-based sorbents were synthesized for binding human IgE. Sorbents effectively removed IgE from human blood plasma. The experimental values of IgE desorption constants were within the range of 1.1 × 10−10 to 1.7 × 10−10 M depending on the orientation of the aptamer on an insoluble matrix. The sorbents were stable during multiple use. Conditions for sorbent regeneration were selected. These chromatographic materials can be used for medical and biotechnological applications.

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Authors and Affiliations

  1. Belozersky Institute of Physicochemical Biology, Moscow State University, Moscow, 119992, Russia

    V. A. Spiridonova

  2. Russian Cardiological Research and Production Complex, Russian Ministry of Healthcare, ul. 3ya Cherepkovskaya 15a, Moscow, 121552, Russia

    P. A. Levashov, E. D. Ovchinnikova, O. I. Afanasieva, I. Yu. Adamova & S. N. Pokrovsky

  3. Department of Chemistry, Moscow State University, Moscow, 119992, Russia

    P. A. Levashov & K. A. Glinkina

Authors
  1. V. A. Spiridonova
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  2. P. A. Levashov
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  3. E. D. Ovchinnikova
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  4. O. I. Afanasieva
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  5. K. A. Glinkina
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  6. I. Yu. Adamova
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  7. S. N. Pokrovsky
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Correspondence to P. A. Levashov.

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Original Russian Text © V.A. Spiridonova, P.A. Levashov, E.D. Ovchinnikova, O.I. Afanasieva, K.A. Glinkina, I.Yu. Adamova, S.N. Pokrovsky, 2014, published in Bioorganicheskaya Khimiya, 2014, Vol. 40, No. 2, pp. 166–169.

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Spiridonova, V.A., Levashov, P.A., Ovchinnikova, E.D. et al. DNA aptamer-based sorbents for binding human IgE. Russ J Bioorg Chem 40, 151–154 (2014). https://doi.org/10.1134/S1068162014020125

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  • DOI: https://doi.org/10.1134/S1068162014020125

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