Journal of Analytical Chemistry

, Volume 73, Issue 3, pp 236–242 | Cite as

A Novel Method for the Rapid Determination of Isonicotinic Hydrazide in Aqueous Solutions Using Reflectance Spectrophotometry and Colorimetry

  • L. V. Zrelova
  • E. I. Belyaeva
  • D. Yu. Marchenko
  • E. A. Ivanova
  • D. A. Sandzhieva
  • A. G. Dedov


A rapid method is developed for the quantitative determination of 5–35 g/L of isonicotinic hydrazide (INH, isoniazide) in aqueous media. The method is based on the formation of copper(I) oxide in the reaction of isonicotinic hydrazide with copper ions on the surface of indicator tablets and the subsequent recording of the analytical signal by reflectance spectrophotometry and colorimetry. Aluminum oxide is proposed as a solid-phase substrate for the production of indicator tablets. Isonicotinic hydrazide can be determined in the tablet form of the Isoniazid preparation.


isoniazid colorimetry indicator tablets analysis of pharmaceutical preparations reflectance spectrophotometry 


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Copyright information

© Pleiades Publishing, Ltd. 2018

Authors and Affiliations

  • L. V. Zrelova
    • 1
  • E. I. Belyaeva
    • 2
  • D. Yu. Marchenko
    • 2
  • E. A. Ivanova
    • 2
  • D. A. Sandzhieva
    • 2
  • A. G. Dedov
    • 2
  1. 1.Sechenov First Moscow State Medical UniversityMoscowRussia
  2. 2.Department of Chemical Technology and EcologyGubkin Russian State University of Oil and GasMoscowRussia

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