Skip to main content
SpringerLink
Log in

Response of antioxidant and osmoprotective systems of wheat seedlings to drought and rehydration

  • Research Papers
  • Published:
Russian Journal of Plant Physiology Aims and scope Submit manuscript

Abstract

Complex study of the effect of soil drought (72 h) and subsequent rehydration for 24 and 48 h on the activities of antioxidant and osmoprotective systems in the leaves of young plants of winter wheat (Triticum aestivum L.) cvs. Ballada (high productivity) and Beltskaya (low productivity) was carried out. Under drought conditions, the content of water in the leaves of cv. Ballada reduced to a lesser degree than in the leaves of cv. Beltskaya. Drought did not affect the rate of leaf growth in cv. Ballada but retarded leaf growth in cv. Beltskaya. Under drought conditions, the content of ascorbate reduced in cv. Beltskaya but was not changed in cv. Ballada; the content of glutathione increased by 19% in cv. Ballada and by 30% in cv. Beltskaya. Under drought conditions, ascorbate peroxidase activity was not changed in cv. Ballada whereas in cv. Beltskaya there was a tendency to its decrease. Glutathione reductase activity in the leaves of cv. Beltskaya increased stronger than in cv. Ballada. Substantial differences between cultivars in the accumulation of reducing sugars and sucrose under water deficit were observed. In both cultivars, drought induced an active proline accumulation. Observed differences in the cultivar responses to water stress evidently indicate differences in the strategy of their adaptation to drought. Drought did not affect the contents of chlorophyll and MDA in both cultivars. The data obtained allow a suggestion that, under conditions of moderate soil drought, the coordinated system of antioxidant defense and osmotic control functioned sufficiently effective; as a result, oxidative stress was not developed in both cultivars. Young plants of both cultivars differing in their responses to water deficit retained the ability to recover after rehydration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

Asc:

ascorbate

APX:

ascorbate peroxidase

GR:

glutathione reductase

References

  1. Chaves, M.M., Flexas, J., and Pinheiro, C., Photosynthesis under Drought and Salt Stress: Regulation Mechanisms from Whole Plant to Cell, Ann. Bot., 2009, vol. 103, pp. 551–560.

    Article  PubMed  CAS  Google Scholar 

  2. Smirnoff, N., The Role of Active Oxygen in the Response of Plant to Water Deficit and Desiccation, New Phytol., 1993, vol. 125, pp. 27–58.

    Article  CAS  Google Scholar 

  3. Gill, S.S. and Tuteja, N., Reactive Oxygen Species and Antioxidant Machinery in Abiotic Stress Tolerance in Crop Plants, Plant Physiol. Biochem., 2010, vol. 48, pp. 909–930.

    Article  PubMed  CAS  Google Scholar 

  4. Foyer, C., Lopez-Delgado, H., Dat, J.F., and Scott, I.M., Hydrogen Peroxide- and Glutathione-Associated Mechanisms of Acclimatory Stress Tolerance and Signaling, Physiol. Plant., 1997, vol. 100, pp. 241–254.

    Article  CAS  Google Scholar 

  5. Sgherri, C.L.M. and Navarri-Izzo, F., Sunflower Seedlings Subjected to Increasing Water Deficit Stress: Oxidative Stress and Defence Mechanisms, Physiol. Plant., 1995, vol. 93, pp. 25–30.

    Article  CAS  Google Scholar 

  6. Srivalli, B., Sharma, G., and Khanna-Chopra, R., Antioxidative Defense System in an Upland Rice Cultivar Subjected to Increasing Intensity of Water Stress Following Recovery, Physiol. Plant., 2003, vol. 119, pp. 509–512.

    Article  Google Scholar 

  7. Polesskaya, O.G., Rastitel’naya kletka i aktivnye formy kisloroda (Plant Cell and Active Oxygen Species), Moscow: Knizhn. Dom Univ., 2007.

    Google Scholar 

  8. Cruz de Carvalho, M.H., Drought Stress and Reactive Oxygen Species. Producing, Scavenging and Signaling, Plant Signal. Behav., 2008, vol. 3, pp. 156–165.

    Article  PubMed  Google Scholar 

  9. Kameli, A. and Lösel, D.M., Carbohydrates and Water Status in Wheat Plants under Water Stress, New Phytol., 1993, vol. 125, pp. 609–614.

    Article  CAS  Google Scholar 

  10. Sánchez, F.J., Manzanares, M., de Andres, E.F., Tenorio, J., and Ayerbe, L., Turgor Maintenance, Osmotic Adjustment and Soluble Sugar and Proline Accumulation in 49 Pea Cultivars in Response to Water Stress, Field Crop Res., 1998, vol. 59, pp. 225–235.

    Article  Google Scholar 

  11. Kuznetsov, Vl.V. and Shevyakova, N.I., Proline under Stress: Biological Role, Metabolism, and Regulation, Russ. J. Plant Physiol., 1999, vol. 46, pp. 274–288.

    CAS  Google Scholar 

  12. Sharma, S.S. and Dietz, K.J., The Significance of Amino Acids and Amino Acid-Derived Molecules in Plant Responses and Adaptation to Heavy Metal Stress, J. Exp. Bot., 2006, vol. 57, pp. 711–726.

    Article  PubMed  CAS  Google Scholar 

  13. Sin’kevich, M.S., Deryabin, A.N., and Trunova, T.I., Characteristics of Oxidative Stress in Potato Plants with Modified Carbohydrate Metabolism, Russ. J. Plant Physiol., 2009, vol. 56, pp. 168–174.

    Article  Google Scholar 

  14. Nikolaeva, M.K., Maevskaya, S.N., Shugaev, A.G., and Bukhov, N.G., Effect of Drought on Chlorophyll Content and Antioxidant Enzyme Activities in Leaves of Three Wheat Cultivars Varying in Productivity, Russ. J. Plant Physiol., 2010, vol. 57, pp. 87–95.

    Article  CAS  Google Scholar 

  15. Goncharova, E.A., Vodnyi status kul’turnykh rastenii i ego diagnostika (Water Status of Cultivated Plants and Its Diagnostics), St. Petersburg: Vses. Inst. Rastenievod., 2005.

    Google Scholar 

  16. Arakawa, N., Tsutsumi, K., Sanceda, N.G., Kurata, T., and Inagari, C., A Rapid and Sensitive Method for the Determination of Ascorbic Acid Using 4,7-Diphenyl-1,10-Phenanthroline, Agric. Biol. Chem., 1981, vol. 45, pp. 1289–1290.

    Article  CAS  Google Scholar 

  17. Anderson, M.E., Determination of Glutathione and Glutathione Disulfide in Biological Samples, Methods Enzymol., 1985, vol. 113, pp. 548–555.

    Article  PubMed  CAS  Google Scholar 

  18. Turkina, M.V. and Sokolova, S.V., Methods for Monosaccharide and Oligosaccharide Determination, Biokhimicheskie metody v fiziologii rastenii (Biochemical Methods in Plant Physiology), Pavlinova, O.A., Ed., Moscow: Nauka, 1971, pp. 7–34.

    Google Scholar 

  19. Bartoli, C.G., Simontacchi, M., Tambussi, E.A., Beltrano, J., Montaldi, E.R., and Puntarulo, S., Drought and Water-Dependent Oxidative Stress: Effect on Antioxidant Content in Triticum aestivum L. Leaves, J. Exp. Bot., 1999, vol. 50, pp. 375–383.

    Article  CAS  Google Scholar 

  20. Herbringer, K., Tausz, M., Wonisch, A., Soja, G., Sorger, A., and Grill, D., Complex Interactive Effects of Drought and Ozone Stress on the Antioxidant Defense System of Two Wheat Cultivars, Plant Physiol. Biochem., 2002, vol. 40, pp. 691–696.

    Article  Google Scholar 

  21. Robinson, J.M. and Bunce, J.A., Influence of Drought-Induced Water Stress on Soybean and Spinach Leaves Ascorbate-Dehydroascorbate Level and Redox Status, Int. J. Plant Sci., 2000, vol. 161, pp. 271–279.

    Article  PubMed  CAS  Google Scholar 

  22. Sigeoka, S., Ishikava, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., and Yoshimura, K., Regulation and Function of Ascorbate Peroxidase Isoenzymes, J. Exp. Bot., 2002, vol. 53, pp. 1305–1319.

    Article  Google Scholar 

  23. Hare, P.D., Cress, W.F., and van Staden, J., Dissecting the Role of Osmolite Accumulation during Stress, Plant Cell Environ., 1998, vol. 21, pp. 535–553.

    Article  CAS  Google Scholar 

  24. Lawlor, D. and Tezara, W., Causes of Decreased Photosynthetic Rate and Metabolic Capacity in Water-Deficient Leaf Cells: A Critical Evaluation of Mechanisms and Integration Processes, Ann. Bot., 2009, vol. 103, pp. 561–579.

    Article  PubMed  CAS  Google Scholar 

  25. Mattioni, C., Lacerenza, N.G., Troccoli, A., De Leonardis, A.M., and Di Fonzo, N., Water and Salt-Induced Alteration in Proline Metabolism of Triticum durum Seedlings, Physiol. Plant., 1997, vol. 101, pp. 787–792.

    Article  CAS  Google Scholar 

  26. Bormotova, T.S., Semenov, O.G., and Kholodova, V.P., Resistance of Spring Wheat Allocytoplasmatic Hybrids to Soil Drought, Vestn. Ross. Univ. Druzhby Narodov, ser. Sel’khoz. Nauki, Agrochimiya, 2004, no. 11, pp. 22–27.

    Google Scholar 

  27. Chen, C. and Dickman, M.B., Proline Suppresses Apoptosis in the Fungal Pathogen Colletotrichum trifolii, Proc. Natl. Acad. Sci. USA, 2005, vol. 102, pp. 3459–3464.

    Article  PubMed  CAS  Google Scholar 

  28. Radyukina, N.L., Shashukova, A.V., Shevyakova, N.I., and Kuznetsov, Vl.V., Proline Involvement in the Common Sage Antioxidant System in the Presence of NaCl and Paraquat, Russ. J. Plant Physiol., 2008, vol. 55, pp. 649–656.

    Article  CAS  Google Scholar 

  29. Shao, H.B., Chu, L.Y., Wu, G., Zhang, J.H., Lu, Z.H., and Hu, Y.C., Changes of Some Anti-Oxidative Physiological Indices under Soil Water Deficits among 10 Wheat (Triticum aestivum L.) Genotypes at Tillering Stage, Colloids Surf. B: Biointerfaces, 2007, vol. 54, pp. 143–149.

    Article  CAS  Google Scholar 

  30. Sgherri, C.L.M., Maffei, M., and Navari-Izzo, F., Antioxidative Enzymes in Wheat Subjected to Increasing Water Deficit and Rewatering, J. Plant Physiol., 2000, vol. 157, pp. 273–279.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    S. N. Maevskaya & M. K. Nikolaeva

Authors
  1. S. N. Maevskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. K. Nikolaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. K. Nikolaeva.

Additional information

Original Russian Text © S.N. Maevskaya, M.K. Nikolaeva, 2013, published in Fiziologiya Rastenii, 2013, Vol. 60, No. 3, pp. 351–359.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Maevskaya, S.N., Nikolaeva, M.K. Response of antioxidant and osmoprotective systems of wheat seedlings to drought and rehydration. Russ J Plant Physiol 60, 343–350 (2013). https://doi.org/10.1134/S1021443713030084

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1021443713030084

Keywords

Search

Navigation