Abstract
Expansins are non-enzymatic plant proteins breaking hydrogen bonds between cellulose microfibrils and hemicellulose polymer matrix. Each plant has many expansin genes, whose protein products participate in the regulation of plant growth and development mainly by regulating cell expansion. To analyze the effects of elevated expansin expression on the plant organ sizes, we cloned the AtEXPA10 gene from Arabidopsis thaliana and PnEXPA1 gene from Populus nigra. Transgenic tobacco plants expressing the target genes were obtained. The obtained transgenic tobacco plants were shown to have significantly larger leaves and longer stems compared to control plants. The flowers were quite insignificantly larger, but at the same time transgenic plants had more flowers. The microscopic studies showed that the organs of AtEXPA10-carrying plants were larger mainly due to stimulated cell proliferation, whereas the overexpression of the PnEXPA1 gene activated cell expansion.
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Abbreviations
- BA:
-
benzyladenine
- Hyg:
-
hygromycin
- MS:
-
Murashige and Skoog nutrient medium
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Original Russian Text © B.R. Kuluev, A.B. Knyazev, Ya.P. Lebedev, A.V. Chemeris, 2012, published in Fiziologiya Rastenii, 2012, Vol. 59, No. 1, pp. 108–117.
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Kuluev, B.R., Knyazev, A.B., Lebedev, Y.P. et al. Morphological and physiological characteristics of transgenic tobacco plants expressing expansin genes: AtEXP10 from Arabidopsis and PnEXPA1 from poplar. Russ J Plant Physiol 59, 97–104 (2012). https://doi.org/10.1134/S1021443712010128
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DOI: https://doi.org/10.1134/S1021443712010128