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Characterization and functional analysis of the PtEXLA1 gene from poplar

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Abstract

Expansin plays a crucial role in plant growth and stress resistance as a cell wall relaxation protein. The expansin family consists of four subfamilies: EXPA, EXPB, EXLA, and EXLB. However, a few reports have been previously published investigating EXLA genes. The research here aimed to characterize the PtEXLA1 gene from a popular species (P. alba × P. glandulosa CV.84K) and evaluate its role through genetic transformation to understand its contribution to plant growth and stress resistance. The results showed that the PtEXLA1 gene was 780 bp in length, encoded 259 amino acids, and had typical characteristics of EXLA. The PtEXLA1 transgenic tobacco plants had a larger corolla in comparison to wild-type plants, and exhibited higher resistance to drought, high temperature, and salt stress based on the evaluation of chlorophyll content, relative conductivity, and malondialdehyde content. PtEXLA1 can be an efficient gene resource for stress resistance breeding of plants.

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All relevant data are available from the corresponding author upon reasonable request.

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Acknowledgements

The research was funded by the National Natural Science Foundation of China (#31870648)

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  1. Jiafu Liu and Yan Wang have contributed equally to this work.

Authors and Affiliations

  1. National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China

    Jiafu Liu, Yan Wang, Lihong Yang, Xue Wang, Junkang Zhang & Jichen Xu

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  1. Jiafu Liu
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  2. Yan Wang
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  3. Lihong Yang
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  4. Xue Wang
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  5. Junkang Zhang
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Correspondence to Jichen Xu.

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Liu, J., Wang, Y., Yang, L. et al. Characterization and functional analysis of the PtEXLA1 gene from poplar. Plant Biotechnol Rep 18, 119–128 (2024). https://doi.org/10.1007/s11816-023-00885-y

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  • DOI: https://doi.org/10.1007/s11816-023-00885-y

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