Skip to main content
SpringerLink
Log in

Tissue Remodeling of Rat Pulmonary Artery in Hypoxic Breathing. I. Changes of Morphology, Zero-Stress State, and Gene Expression

  • Published:
Annals of Biomedical Engineering Aims and scope Submit manuscript

Abstract

The remodeling of the pulmonary arterial tissue in response to a step change of the oxygen concentration in the gas in which a rat lives was recorded as function of time and function of O2 concentration. Three steps of changing from 20.9% to 17.2%, 13.6%, and 10% O2 were imposed. Earlier work in our laboratory has shown that pulmonary arterial tissue remodeling is significant in the first 24 h after a step change of oxygen tension. Hence we made measurements in this period. Furthermore, data were obtained for tissue remodeling of circumferential and axial lengths of the pulmonary arteries. We recorded the activities of gene expressions in the lung tissues by microarray, determined the dose response curves of gene expression in the homogenized whole lungs with respect to four levels of O2 concentration, and obtained the time courses of gene expression in the lung parenchyma in 30 days after a step decrease of O2 concentration from 20.9% to 10%. We would like to suggest that the correlation of gene expression with physiological function parameters, i.e., time, O2 tension, blood pressure, opening angle, wall thicknesses, etc., is the way to narrow down the search for specific genes for specific physiological functions. © 2001 Biomedical Engineering Society.

PAC01: 8719Uv

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucl. Acids Res.25:3389–3402, 1997.

    Article  PubMed  Google Scholar 

  2. Benson, D. A., M. S. Boguski, D. J. Lipman, and J. Ostell. GenBank. Nucl. Acids Res.25:1–6, 1977.

    Google Scholar 

  3. Chen, J. J. W., R. Wu, P. C. Yang, J. Y. Huang, Y. P. Sher, M. H. Han, W. C. Kao, P. J. Lee, T. F. Chiu, F. Chang, Y. W. Chu, C. W. Wu, and K. Peck. Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection. Genomics51:313–324, 1998.

    Google Scholar 

  4. Crystal, R. G., J. B. West, E. R. Weibel, and P. J. Barnes (eds.). The Lung: Scientific Foundations, 2nd ed. Philadelphia: Lippincott-Raven, 1997, p. 2811.

    Google Scholar 

  5. Fung, Y. C. Biodynamics: Circulation. New York: Springer, 1984, pp. 54–68.

    Google Scholar 

  6. Fung, Y. C., and S. Q. Liu. Changes of zero-stress state of rat pulmonary arteries in hypoxic hypertension. J. Appl. Physiol.70:2455–2470, 1991.

    Google Scholar 

  7. Grover, R. F., W. W. Wagner, Jr., I. F. McMurtry, and J. T. Reeves. Pulmonary circulation. In: Handbook of Physiology, Sec. 2: The Cardiovascular System, Vol. III, part 1, Chap. 4, edited by J. T. Shepherd, and F. M. Abboud. Bethesda: American Physiological Society, 1983, pp. 103–136.

    Google Scholar 

  8. Huang, W., D. Delgado-West, J. T. Wu, and Y. C. Fung. Tissue remodeling of rat pulmonary artery in hypoxic breathing. II. Course of change of mechanical properties. Ann. Biomed. Eng.29:552–562 (2001).

    Google Scholar 

  9. Huang, W., Z. Shen, N. E. Huang, and Y. C. Fung. Engineering analysis of biological variables: An example of blood pressure over 1 day. Proc. Natl. Acad. Sci. U.S.A.95:4816–4821, 1998.

    Google Scholar 

  10. Huang, W., Z. Shen, N. E. Huang, and Y. C. Fung. Use of intrinsic modes in biology: Examples of indicial response of pulmonary blood pressure to step hypoxia. Proc. Natl. Acad. Sci. U.S.A.95:12766–12771, 1998.

    Google Scholar 

  11. Huang, W., Z. Shen, N. E. Huang, and Y. C. Fung. Nonlinear indicial response of complex nonstationary oscillations as pulmonary hypertension responding to step hypoxia. Proc. Natl. Acad. Sci. U.S.A.96:1834–1839, 1999.

    Google Scholar 

  12. Jiang, Z. L., G. S. Kassab, and Y. C. Fung. Diameter-defined Strahler system and connectivity matrix of the pulmonary arterial tree. J. Appl. Physiol.76:882–892, 1994.

    Google Scholar 

  13. Lennon, G. G., C. Auffray, M. Polymeropoulos, and M. B. Soares. The I.M.A.G.E. Consortium: An integrated molecular analysis of genomes and their expression. Genomics33:151–152, 1996.

    PubMed  Google Scholar 

  14. Liu, S. Q., and Y. C. Fung. Influence of STZ-induced diabetes on zero-stress states of rat pulmonary and systemic arteries. Diabetes41:136–146, 1992.

    Google Scholar 

  15. Liu, S. Q., and Y. C. Fung. Changes in the rheological properties of blood vessel tissue remodeling in the course of development of diabetes. Biorheology29:443–457, 1992.

    Google Scholar 

  16. Liu, S. Q., and Y. C. Fung. Material coefficients of the strain energy function of pulmonary arteries in normal and cigarette smoke-exposed rats. J. Biomech.26:1261–1269, 1993.

    Google Scholar 

  17. Liu, S. Q., and Y. C. Fung. Material coefficients of the strain energy function of pulmonary arteries in normal and cigarette smoke-exposed rats. J. Biomech.26:1261–1269, 1993.

    Google Scholar 

  18. Meyrick, B., and L. Reid. The effect of continued hypoxia on rat pulmonary arterial circulation: An ultrastructural study. Lab. Invest.38:188–200, 1978.

    Google Scholar 

  19. Schuler, G. D., et al.A gene map of the human genome. Science274:540–546, 1996.

    PubMed  Google Scholar 

  20. Schulman, D. S., and R. A. Matthay. The right ventricle in pulmonary disease. Cardiol. Clin.10:111–138, 1992.

    Google Scholar 

  21. Sobin, S. S., H. M. Tremer, J. D. Hardy, and H. P. Chiodi. Changes in arteriole in acute and chronic hypoxic pulmonary hypertension and recovery in rat. J. Appl. Physiol.55:1445–1455, 1983.

    PubMed  Google Scholar 

  22. Sobin, S. S., and P. C. Y. Chen. Vascular cylindricity in animals and plants. Ann. Biomed. Eng.25:S-32, 1997.

    Google Scholar 

  23. Tamayo, P., D. Slonim, J. Mesirov, Q. Zhu, S. Kitareewan, E. Dmitrovsky, E. S. Lander, and T. R. Golub. Interpreting patterns of gene expression with self-organizing maps: Methods and application to hematopoietic differentiation. Proc. Natl. Acad. Sci. U.S.A.96:2907–2912, 1999.

    PubMed  Google Scholar 

  24. Vaishnav, R. N., and J. Vossoughi. Estimation of residual strains in aortic segments. Biomedical Engineering, II, Recent Developments, edited by C. W. Hall. New York: Pergamon, 1983, pp. 330–333.

    Google Scholar 

  25. Voelkel, N. F., R. M. Tuder, and E. K. Weir. Pathophysiology of primary pulmonary hypertension: From physiology to molecular mechanisms. In: Primary Pulmonary Hypertension, edited by L. J. Rubin and S. Rich. New York: Marcel Dekker, 1997, pp. 83–133.

    Google Scholar 

  26. von Euler, V. S., and G. Liljestrand. Observations on the pulmonary arterial blood pressure in the cat. Acta Physiol. Scand.12:301–320, 1946.

    Google Scholar 

  27. Wagner, W. W., Jr., and E. K. Weir. The Pulmonary Circulation and Gas Exchange. Armonk: Futura, 1994, p. 424.

    Google Scholar 

  28. Ward, M. P., J. B. West, and J. S. Milledge. High Altitude Medicine and Physiology, 2nd ed. New York: Chapman and Hall, 1995, p. 618.

    Google Scholar 

  29. West, J. B. High Life: A History of High-Altitude Physiology and Medicine. New York: Oxford University Press, 1998, p. 493.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Bioengineering, University of California San Diego, La Jolla, CA

    Wei Huang, David Delgado-West, Jonathan T. Wu & Yuan Cheng Fung

  2. Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan

    Yuh-Pyng Sher & Konan Peck

Authors
  1. Wei Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuh-Pyng Sher
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Delgado-West
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jonathan T. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Konan Peck
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yuan Cheng Fung
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, W., Sher, YP., Delgado-West, D. et al. Tissue Remodeling of Rat Pulmonary Artery in Hypoxic Breathing. I. Changes of Morphology, Zero-Stress State, and Gene Expression. Annals of Biomedical Engineering 29, 535–551 (2001). https://doi.org/10.1114/1.1380416

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1114/1.1380416

Search

Navigation