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Establishment and characterization of a novel Philadelphia-chromosome positive chronic myeloid leukemia cell line, TCC-S, expressing P210 and P190 BCR/ABL transcripts but missing normal ABL gene

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Abstract

A novel Philadelphia-chromosome positive (Ph+) cell line, TCC-S, has been established from a patient with Ph+ chronic myeloid leukemia (CML) in the blastic crisis. TCC-S cells were shown to express both P210 and P190 BCR/ABL transcripts by reverse transcriptase-polymerase chain reaction (PCR), although quantitative-PCR revealed that TCC-S cells mainly expressed P210 BCR/ABL transcript. Karyotype analysis revealed several triploid clones which constantly harbored two der(9)del(9)(p12)t(9;22)(q34;q11)s and two del(9)(q21)s. The der(9)del(9)(p12)t(9;22)(q34;q11) is rarely found in other CML cell lines. Moreover, to the best of our knowledge, del(9)(q21) resulting in missing of a restrict region including normal ABL gene has not been found among CML cell lines previously described. Thus, TCC-S cells with only BCR/ABL gene and no normal ABL gene may be a useful tool for functional study of ABL in Ph+ CML.

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Authors and Affiliations

  1. Division of Ultrafine Structure, Department of Pathology, Research Institute, International Medical Center of Japan, Toyama 1-21-1, Shinjuku-Ku, 162-0052, Tokyo, Japan

    Phan Nguyen Van Thanh, Phan Thi Xinh & Yuko Sato

  2. Division of Hematology and Medical Oncology, Tochigi Cancer Center, Tochigi, Japan

    Yasuhiko Kano

  3. Department of Human Genetics, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

    Phan Nguyen Van Thanh, Phan Thi Xinh & Katsushi Tokunaga

Authors
  1. Phan Nguyen Van Thanh
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  2. Phan Thi Xinh
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  3. Yasuhiko Kano
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  4. Katsushi Tokunaga
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  5. Yuko Sato
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Correspondence to Yuko Sato.

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Van Thanh, P.N., Xinh, P.T., Kano, Y. et al. Establishment and characterization of a novel Philadelphia-chromosome positive chronic myeloid leukemia cell line, TCC-S, expressing P210 and P190 BCR/ABL transcripts but missing normal ABL gene. Hum Cell 18, 25–33 (2005). https://doi.org/10.1111/j.1749-0774.2005.tb00054.x

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  • DOI: https://doi.org/10.1111/j.1749-0774.2005.tb00054.x

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