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Purification of serine carboxypeptidase from the hepatopancreas of Japanese common squid Todarodes pacificus and its application for elimination of bitterness from bitter peptides

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Abstract

An acidic serine carboxypeptidase (CPase Tpa) from the hepatopancreas of Japanese common squid Todarodes pacificus was purified. Purified CPase Tpa had a molecular mass of 36 kDa on sodium dodecylsulfate-polyacrylamide gel electrophoresis, and an isoelectric point of 6.0. The optimum pH of CPase Tpa was pH 4.0. In investigating the specificity of CPase Tpa for several peptide substances, it was found that peptides with hydrophobic or bulky amino acid residues at the P1 position reacted well. The enzymatic activity was almost completely inhibited by p-chloromercuribenzoic acid, monoiodoacetic acid, diisopropylfluorophosphate and HgCl2. This is the basis for its grouping in the serine carboxypeptidase family (EC 3. 4. 16. 5). The substrate specificity of CPase Tpa can be used to eliminate the bitterness of bitter peptides. In this study, the bitterness-reductive effect using bitter peptides prepared by hydrolyzing soy protein, casein and corn gluten with pepsin or trypsin was tested. The bitterness of soy peptide digested with pepsin was completely eliminated by treatment with CPase Tpa, whereas the bitterness of casein digested with trypsin and corn peptide digested with pepsin were somewhat less efficient. On the basis of these results, it is anticipated that CPase Tpa would be effective in eliminating the bitterness of some bitter peptides.

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Authors and Affiliations

  1. Technical Research Center, T. Hasegawa Co. Ltd, Nakahara-ku, 211-0022, Kawasaki, Kangawa, Japan

    Tsuyoshi Komai, Choko Kawabata, Hiroaki Tojo & Shinobu Gocho

  2. Laboratory of Molecular Enzymology, Department of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, 192-8577, Tokyo, Japan

    Eiji Ichishima

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  1. Tsuyoshi Komai
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  2. Choko Kawabata
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  3. Hiroaki Tojo
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  4. Shinobu Gocho
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  5. Eiji Ichishima
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Correspondence to Tsuyoshi Komai.

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Komai, T., Kawabata, C., Tojo, H. et al. Purification of serine carboxypeptidase from the hepatopancreas of Japanese common squid Todarodes pacificus and its application for elimination of bitterness from bitter peptides. Fish Sci 73, 404–411 (2007). https://doi.org/10.1111/j.1444-2906.2007.01348.x

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  • DOI: https://doi.org/10.1111/j.1444-2906.2007.01348.x

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