Abstract
In order to investigate the relationships between levels of lipid peroxides (LPO), hydroxy lipids (L-OH) and of antioxidative enzymes in live fish, cultured cells from tilapia liver (Hepa-T1) were treated with oxidative stressors, 2,2′-azobios(2-amidino-propane) dihydrochloride (AAPH) and H2O2. By treating with 5 mM AAPH, LPO and L-OH of the cells significantly increased time-dependently. In particular, L-OH increased from 7.6±0.7 to 23.6±1.8 nmol/mg protein after 2 h of treatment, and synchronously the glutathione peroxidase (GPx) activity of the cells increased from 259±106 to 1970±135 mU/mg protein. In the case of H2O2, however, no elevation of L-OH was observed. In this case, catalase (CAT) and superoxide dismutase (SOD) activities increased time-dependently. Especially, after 2 h exposure, the CAT activity increased remarkably from 217±21 to 2510±120 mU/mg protein, though the GPx activity did not change significantly. These results suggest that the elevation of the L-OH level is closely associated with the elevation of GPx activity, but not with that of CAT or SOD. However, when vitamin E-enriched Hepa-T1 cells were treated with AAPH and H2O2, the production of LPO and L-OH was suppressed significantly. The findings suggest that vitamin E also plays an important role in the levels of the oxidation products in vivo.
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15 June 2018
"The original article was corrected. We had used a cell line, Hepa-T1, in the article as a cell line derived from tilapia liver. However, in April 2018 the provider of Hepa-T1, RIKEN BioResource Research Center (RIKEN BRC), informed us that Hepa-T1 was derived from eel but not tilapia. RIKEN BRC found this misidentification by DNA barcoding of the mitochondrial gene cytochrome c oxidase subunit 1 (COI) (Fig.��1) in a course of pursuing more precise method for species identification of cell lines (Almeida et al. 2016). RIKEN BRC suggested a possibility that Hepa-T1 was replaced with Hepa-E1 cell line which was derived from eel and was deposited from the same scientist. RIKEN BRC accepted the deposition of these two cell lines simultaneously in March 1995 and could not detect this misidentification by the method available at that time, i.e. isozyme analysis with lactate dehydrogenase and nucleotide phosphorylase."
15 June 2018
"The original article was corrected. We had used a cell line, Hepa-T1, in the article as a cell line derived from tilapia liver. However, in April 2018 the provider of Hepa-T1, RIKEN BioResource Research Center (RIKEN BRC), informed us that Hepa-T1 was derived from eel but not tilapia. RIKEN BRC found this misidentification by DNA barcoding of the mitochondrial gene cytochrome c oxidase subunit 1 (COI) (Fig.��1) in a course of pursuing more precise method for species identification of cell lines (Almeida et al. 2016). RIKEN BRC suggested a possibility that Hepa-T1 was replaced with Hepa-E1 cell line which was derived from eel and was deposited from the same scientist. RIKEN BRC accepted the deposition of these two cell lines simultaneously in March 1995 and could not detect this misidentification by the method available at that time, i.e. isozyme analysis with lactate dehydrogenase and nucleotide phosphorylase."
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Tanaka, R., Hatate, H., Ito, M. et al. Elevation of lipid peroxide level and production of hydroxy lipids in cultured Hepa-T1 cells by oxidative stressors. Fish Sci 72, 665–672 (2006). https://doi.org/10.1111/j.1444-2906.2006.01197.x
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DOI: https://doi.org/10.1111/j.1444-2906.2006.01197.x