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Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba

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Summary

In an attempt to increase productivity, the effects of the elicitors methyl jasmonate (MJ) and salicylic acid (SA) on the production of bilobalide (B), ginkgolide A (GA), and ginkgolide B (GB) were studied in cell suspension cultures of Ginkgo biloba. MJ treatments increased the amounts of B, GA, and GB, concomitant with a slight decrease in cell growth. After treatment of 0.01 mM MJ, levels of GA and GB increased 4.3-and 8.2-fold over controls by 12 h and declined after 24h. The 1.0mM MJ treatment produced a maximal release of B after 12h of exposure and increased the concentration of B in the culture medium up to 6.25-fold compared with the controls. Treatment with 1.0mM SA transiently enhanced GA and GB production up to 3.1-and 6.1-fold, respectively, compared with the control. However, treatment 1.0 mM SA did not have a significant effect on B production. When treated with 0.01 mM SA, the level of B in the cells was increased 5.4-fold over controls by 12h and declined after 24h. The concentrations and exposure times of both MJ and SA were factors that strongly affected the production of B, GA, and GB. The results from this study suggest that MJ and SA directly or indirectly increased the production of B, GA, and GB in cells, and stimulated the release of these metabolites into the culture medium.

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References

  • Aerts, R. J.; Schafer, A.; Hesse, M.; Baumann, T. W.; Slusarenko A. Signalling molecules and the synthesis of alkaloids in Catharanthus roseus seedlings. Phytochemistry 42:417–422; 1996.

    Article  CAS  Google Scholar 

  • Carrier, D. J.; van Beek, T. A.; van der Heijden, R.; Veroorte, R. Distribution of ginkgolides and terpenoids biosynthetic activity in Ginkgo biloba. Phytochemistry 48:89–92; 1998.

    Article  CAS  Google Scholar 

  • Cosio, E. G.; Frey, T.; Verduyn, R.; van Boom, J.; Ebel, J. High-affinity binding of a synthetic heptaglucoside and fungal glucan phytoalexin elicitors to soybean membranes. FEBS Lett. 271:223–226; 1990.

    Article  PubMed  CAS  Google Scholar 

  • Draper, J. Salicyliate, superoxide synthesis and cell suicide in plant defense. Trends Plant Sci. 2:162–165; 1997.

    Article  Google Scholar 

  • Durner, J.; Shah, J.; Klessig, D. F. Salicylic acid and disease resistance in plants. Trends Plant Sci. 2:1360–1385; 1997.

    Article  Google Scholar 

  • Fu, X. O.; Lu, D. L. Stimulation of shikonin production by combined fungal elicitation in situ extraction in suspension cultures of Arnebia euchroma. Enzyme Microbiol. Technol. 24:243–246; 1999.

    Article  CAS  Google Scholar 

  • Gergorio, G. H.; Victor, M. L. V. Effect of acetylsalicylic acid on secondary metabolism of Catharanthus roseus tumor suspension cultures. Plant Cell Rep. 16:287–290; 1997.

    Google Scholar 

  • Kang, S. M.; Jung, H. Y.; Kang, Y. M.; Yun, D. J.; Bahk, J. D.; Yang, J. K.; Choi, M. S. Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitions root cultures of Scopolia parviflora. Plant Sci. 166:745–751; 2004.

    Article  CAS  Google Scholar 

  • Ketchum, R. E. B.; Gibson, D. M.; Croteau, R. B.; Shuler, M. L. The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol. Bioeng. 62:97–105; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Kim, G. S.; Paek, Y. W.; Ko, K. M.; Hwang, S. J.; Kim, Y. J.; Chung, S. J.; Hwang, B. Detection of flavonoid compounds by cell culture of Ginkgo biloba L. Korean J. Biotechnol. Bioeng. 11:1–7; 1996.

    Google Scholar 

  • Klessing, D. F.; Malamy, J. The salicylic acid signal in plants. Plant Mol. Biol. 26:1439–1458; 1994.

    Article  Google Scholar 

  • Laurain, D.; Guiller, J. T.; Chenieux, J. C.; van Beek, T. A. Production of ginkgolide and bilobalide in transformed and gametophyte derived cell cultures of Ginkgo biloba. Phytochemistry 46:127–130; 1997.

    Article  CAS  Google Scholar 

  • Lois, R.; Dietrich, A.; Hahlbrock, K.; Schulz, W. A phenylanine ammonialyase gene from parsley: structure, regulation and identification of elicitor and light responsive cis-acting elements. EMBO J. 8:1641–1648; 1989.

    PubMed  CAS  Google Scholar 

  • Manthe, B.; Schulz, M.; Schnabl, H. Effects of salicylic acid on growth and stomatal movements of Vicia faba L. evidence for salicylic acid metabolization. J. Chem. Ecol. 18:1525–1539; 1992.

    Article  CAS  Google Scholar 

  • Mehmetoglu, U.; Curtis, W. R. Effects of abiotic inducers on sesquiterpene synthesis in hairy root and cell-suspension cultures of Hyoscyamus muticus. Appl. Biochem. Biotechnol. 67:71–77; 1997.

    CAS  Google Scholar 

  • Menke, F. L.; Parchmann, S.; Mueller, M. J.; Kijne, J. W.; Memelink, J. Involvement of the octadecanoid pathway and protein phosphorylation in fungal elicitor-induced expression of terpenoid indole alkaloid biosynthelic gene in Catharanthus roseus. Plant Physiol. 119:1289–1296; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Mizukami, H.; Tavira, Y.; Ellis, B. E. Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Rep. 12:706–709; 1993.

    Article  CAS  Google Scholar 

  • Neumann, D. G.; Krauss, G.; Hieke, M.; Groger, D. Indole alkaloid formation and storage in cell suspension cultures of Catharanthus roseus. Plant Med. 48:20–23; 1983.

    CAS  Google Scholar 

  • Sim, S. J.; Chang, H. N.; Liu, J. R.; Jung, K. C. Production and secretion of indole alkaloids by cultures of Catharanthus roseus hairy root: effect of in situ adsorption, permeabilization and fungal elicitor. J. Ferment. Bioeng. 78:229–234; 1994.

    Article  CAS  Google Scholar 

  • Smith, P. F.; Maclennan, K.; Darlington, C. L. The neuroprotective properties of the Ginkgo biloba leaf: a review of the possible relationship to platelet-activating factor (PAF). J. Ethnopharmacol. 50:131–139; 1996.

    Article  PubMed  CAS  Google Scholar 

  • Song, S. H.; Byun, S. Y. Elicitation of camptothecin production in cell cultures of Camptotheca acuminata. Biotechnol. Bioprocess Eng. 3:91–95; 1998.

    Article  Google Scholar 

  • Van Beek, T. A.; Bombardelli, E.; Morazzoni, P.; Peterlongo, F. Ginkgo biloba L. Fitoterapia 69:195–244; 1998.

    Google Scholar 

  • Wang, C.; Wu, J.; Mei, X. Enhancement of taxol production and excretion in Taxus chinensis cell culture by fungal elicitation and medium renewal. Appl. Microbiol. Biotechnol. 55:404–410; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Zhao, J.; Zhu, W. H.; Hu, Q.; Guo, Y. Q. Elicitor-induced indole alkaloid biosynthesis in Catharanthus roseus cell cultures is related to Ca2+ influx and the oxidative burst.Plant Sci. 161:423–431; 2001.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Forest Science, Gyeongsang National University, 660-901, Jinju, Korea

    Seung-Mi Kang, Ji-Yun Min, Yong-Duck Kim, Dong-Jin Park & Ha-Na Jung

  2. Department of Forestry, Mississippi State University, P.O. Box 9681, 39762, MS

    Young-Min Kang

  3. Environmental Biotechnology Research Center, Gyeongsang National University, 660-701, Jinju, Korea

    Seon-Won Kim & Myung-Suk Choi

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  1. Seung-Mi Kang
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  2. Ji-Yun Min
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  3. Yong-Duck Kim
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  4. Young-Min Kang
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  5. Dong-Jin Park
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  6. Ha-Na Jung
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  8. Myung-Suk Choi
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Correspondence to Myung-Suk Choi.

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Kang, SM., Min, JY., Kim, YD. et al. Effects of methyl jasmonate and salicylic acid on the production of bilobalide and ginkgolides in cell cultures of Ginkgo biloba . In Vitro Cell.Dev.Biol.-Plant 42, 44–49 (2006). https://doi.org/10.1079/IVP2005719

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  • DOI: https://doi.org/10.1079/IVP2005719

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