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Inhibitory effects of filter-sterilized media on microspore development and embryogenesis in Brassica napus and Triticum aestivum

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Summary

Plant tissue culture media, sterilized through commercial filter membrane units, inhibited plant cell growth and development. Embryogenesis was suppressed in microspore cultures of Brassica napus and anther cultures of Triticum aestivum, and in vitro pollen development was suppressed in B. napus. Inhibition of growth and development occurred on media that had been filter-sterilized in low volumes through non-washed filter membrane units of all brands tested, including Nalgene, Corning, Millipore, and Sartorius membrane products. Similar results were obtained with Costar Transwells and Millipore inserts (Millicell-CM.-HA, and-PCF). These deleterious effects were eliminated by washing the membranes with water followed by a small volume of the medium. Media sterilized by filtration through washed filters produced significant improvements of growth and development of embryos and pollen.

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Authors and Affiliations

  1. Department of Biology, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    J. -P. Zhao, C. Gervais & W. Newcomb

  2. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, Building 21, K1A 0C6, Ottawa, Ontario, Canada

    J. Simmonds & D. H. Simmonds

Authors
  1. J. -P. Zhao
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  2. C. Gervais
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  3. J. Simmonds
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  4. W. Newcomb
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  5. D. H. Simmonds
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Correspondence to D. H. Simmonds.

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Zhao, J.P., Gervais, C., Simmonds, J. et al. Inhibitory effects of filter-sterilized media on microspore development and embryogenesis in Brassica napus and Triticum aestivum . In Vitro Cell.Dev.Biol.-Plant 41, 281–284 (2005). https://doi.org/10.1079/IVP2005639

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  • DOI: https://doi.org/10.1079/IVP2005639

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