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Plant regeneration from leaf petioles in Camptotheca acuminata

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Summary

Camptothecin, produced by Camptotheca acuminata, is a pharmaceutically important compound. Transgenic technology has potential uses for the enhancement of camptothecin production; however, an efficient plant regeneration protocol for C. acuminata is not currently available. Factors that affected successful seedling germination were evaluated. The regeneration potential of various parts of seedlings was tested. Camptothecin production in regenerated plants was compared to its production in calluses and the original seedlings. Dark incubation and seed coat removal led to a higher germination rate and a higher survival rate after germination. The best shoot induction medium was found to be Gamborg's B5 medium+8.9 μM benzyladenine. Among the calluses induced from various parts of seedlings, leaf petiole calluses, leaf dise calluses, and cotyledon calluses regenerated shoots, but internode calluses did not. Furthermore, leaf petiole calluses and leaf dise calluses regenerated normal shoots, while cotyledon calluses regenerated hyperhydric shoots. Moreover, leaf petiole calluses had a higher shoot regeneration rate, 50% versus 9%, and a higher shoot number, 6.2±0.5 versus 2.0±0.3, than did leaf dise calluses on the best shoot induction medium. It took 4–6 wk to regenerate shoots after transfer into shoot induction media. Camptothecin concentration in the regenerated plants was significantly higher than that in the calluses and similar to that in the original seedlings. In conclusion, leaf petioles provide efficient plant regeneration of C. acuminata.

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Author notes

  1. Zhanhai Li

    Present address: Department of Biostatisties and Medical Informatics, University of Wisconsin-Madison, 53792-4675, Madison, WI

Authors and Affiliations

  1. School of Renewable Natural Resources, Louisiana State University Agricultural Center, 70803, Baton Rouge, LA

    Zhanhai Li & Zhijun Liu

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  1. Zhanhai Li
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  2. Zhijun Liu
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Correspondence to Zhanhai Li.

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Li, Z., Liu, Z. Plant regeneration from leaf petioles in Camptotheca acuminata . In Vitro Cell.Dev.Biol.-Plant 41, 262–265 (2005). https://doi.org/10.1079/IVP2004631

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  • DOI: https://doi.org/10.1079/IVP2004631

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