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Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula

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Summary

This study investigated the role of antioxidants and amino acids on somatic embryogenesis using the vegetative shoot apices of three genotypes of mature Pinus patula trees. Apart from dithiothreitol at 0.1%, pretreatment of explants or incorporation of antioxidants in the basal nutrient medium had a negative effect on the initiation of embryogenic cultures, somatic embryo production, and plantlet recovery. Inclusion of an amino acid solution mixture, at a concentration of 10.0 mM, in the maturation medium tended to increase somatic embryo production.

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References

  • Anderson, W. C. Propagation of rhododendrons by tissue culture. I. Development of a culture medium for multiplication of shoots. Comb. Proc. Int. Plant. Prop. Soc. 25:129–135; 1975.

    Google Scholar 

  • Barrett, J. D.; Park, Y. S.; Bonga, J. M. The effectiveness of various nitrogen sources in white spruce (Picea glauca (Moench) Voss) somatic embryogenesis. Plant Cell Rep. 16:411–415; 1997.

    CAS  Google Scholar 

  • Benson, E. E. Do free radicals have a role in plant tissue culture recalcitrance?. In Vitro Cell Dev. Biol. Plant 36:163–170; 2000.

    CAS  Google Scholar 

  • Benson, E. E.; Lynch, P. T.; Jones, J. The use of the iron chelating agent desferrioxamine in rice cell cryopreservation: a novel approach for improving recovery. Plant Sci. 110:249–258; 1995.

    Article  CAS  Google Scholar 

  • Elmore, H. W.; Samples, B.; Sharma, S.; Harrison, M. Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture media. Plant Cell Tiss. Organ Cult. 20:131–135; 1990.

    Article  CAS  Google Scholar 

  • Fierer, R. P. The biochemistry of conifer embryo development: amino acids, polyamines and storage proteins. In: Jain, S.; Gupta, P. K.; Newton, R., eds. Somatic embryogenesis in woody plants. Dordrecht: Kluwer Academic Publishers; 1995:317–335.

    Google Scholar 

  • Garin, E.; Bernier-Cardou, M.; Isabel, N.; Klimaszewska, K.; Plourde, A. Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations. Plant Cell Tiss. Organ Cult. 62:27–37; 2000.

    Article  CAS  Google Scholar 

  • Gupta, P. K.; Durzan, D. J. Shoot multiplication from mature trees of Douglas fir and sugar pine. Plant Cell Rep. 4:177–179; 1985.

    Article  CAS  Google Scholar 

  • Jones, J. B.; Murashige, T. Tissue culture propagation of Aechmea fasciata Baker and other Bromeliads. Comb. Proc. Int. Plant. Prop. Soc. 24:117–126; 1974.

    Google Scholar 

  • Khlifi, S.; Tremblay, F. M. Maturation of black spruce somatic embryos. Part I. Effect of L-glutamine on the number and germinability of somatic embryos. Plant Cell Tiss. Organ Cult. 41:23–32; 1995.

    Article  CAS  Google Scholar 

  • Malabadi, R. B.; van Staden, J. Somatic embryos can be induced from the vegetative shoot apex of mature Pinus patula trees. S. Afr. J. Bot. 69:450–451; 2003.

    Google Scholar 

  • Malabadi, R. B.; van Staden, J. Somatic embryogenesis from vegetative shoot apices of mature trees of Pinus patula. Tree Physiol. 25:11–16; 2005.

    PubMed  Google Scholar 

  • Mante, S.; Tepper, H. B. Production of Musa textiles Nee plants from apical meristem slices in vitro. Plant Cell Tiss. Organ Cult. 2:151–159; 1983.

    Article  Google Scholar 

  • Murthy, B. N. S.; Singh, R. P.; Saxena, P. K. Induction of high-frequency somatic embryogenesis in geranium (Pelorgonium × hortorum Bailey cv. Ringo Rose) cotyledonary cultures. Plant Cell Rep. 15:423–426; 1996.

    Article  CAS  Google Scholar 

  • Muyuan, Z.; Abing, X.; Miaobao, Y.; Chunnong, H.; Zhilong, Y.; Linji, W.; Jianjun, Y. Effects of amino acids on callus differentiation in barley anther culture. Plant Cell Tiss. Organ Cult. 22:201–204; 1990.

    Article  Google Scholar 

  • Nichol, J. W.; Slade, D.; Viss, V.; Stuart, D. A. Effect of organic acid pretreatment on the regeneration and development (conversion) of whole plants from callus cultures of alfalfa, Medicago sativa L. Plant Sci. 79:181–192; 1991.

    Article  CAS  Google Scholar 

  • Ohkatsu, Y.; Kajiyama, T.; Ari, Y. Antioxidant activities of tocopherols. Poly. Deg. Stab. 72:303–311; 2001.

    Article  CAS  Google Scholar 

  • Ouedraogo, J. T.; St-Pierre, C. A.; Collin, J.; Rioux, S.; Comeau, A. Effect of amino acids, growth regulators and genotype on androgenesis in barley. Plant Cell Tiss. Organ. Cutl. 53:59–66; 1998.

    Article  CAS  Google Scholar 

  • Salin, M. L.; Bridges, S. M. Chemiluminescence in wounded root tissue. Plant Physiol. 67:43–46; 1981.

    Article  PubMed  CAS  Google Scholar 

  • Smith, D. R. Growth medium for plant embryogenic tissue. Australia Canada Patent #PM5232; 1994.

  • Smith, D. R. The role of in vitro methods in pine plantation establishment: the lesson from New Zealand. Plant Tiss. Cult. Biotechnol. 3:63–73; 1997.

    Article  Google Scholar 

  • Stuart, D. A.; Strickland, S. G. Somatic embryogenesis from cell cultures of Medicago sativa L. I. The role of amino acid additions to the regeneration medium. Plant Sci. Lett. 34:165–174; 1984.

    Article  CAS  Google Scholar 

  • Taji, A.; Williams, R. Tissue culture of Australian plants. Amidale, NSW: University of New England; 1996.

    Google Scholar 

  • Teasdale, R. D. Germination of sustainable Pinus radiata cell suspension culture and studies of cellular nitrogen nutrition. NZ J. For. Sci. 16:377–386; 1986.

    Google Scholar 

  • Thomas, P.; Ravindra, M. B. Shoot-tip culture in mango: influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. J. Hort. Sci. 72:713–722; 1997.

    Google Scholar 

  • Thompson, J. E.; Legge, R. L.; Barber, R. F. The role of free radicals in senescence and wounding. New Phytol. 105:317–344; 1987.

    Article  CAS  Google Scholar 

  • Trottier, M. C.; Collin, J.; Comeau, A. Comparison of media for their aptitude in wheat anther culture. Plant Cell Tiss. Organ Cult. 35:59–67; 1993.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Research Centre for Plant Growth and Development, School of Botany and Zoology, University of KwaZulu-Nata Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa

    Ravindra B. Malabadi & Johannes Van Staden

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  1. Ravindra B. Malabadi
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  2. Johannes Van Staden
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Correspondence to Johannes Van Staden.

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Malabadi, R.B., Van Staden, J. Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula . In Vitro Cell.Dev.Biol.-Plant 41, 181–186 (2005). https://doi.org/10.1079/IVP2004623

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  • DOI: https://doi.org/10.1079/IVP2004623

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