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Acetylsalicylic acid and ammonium-induced somatic embryogenesis and enhanced plumbagin production in suspension cultures of Plumbago rosea L.

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Summary

Somatic embryogenesis was induced from suspension cultures (derived from leaf callus) of an important medicinal plant, Plumbago rosea L. While acetylsalicylic acid (ASA) alone induced embryogenesis, indole-3-acetic acid (IAA) failed to elicit a similar response. This is the first time that ASA-induced somatic embryogenesis has been reported in cultured cells. Optimal embryogenic response per culture was observed in Murashige and Skoog’s medium containing a combination of ASA (8.32 μM) and IAA (5.06 μM). but 1-naphthaleneacetic acid and indole-3-butyric acid individually did not induce somatic embryogenesis. Increase in the concentration of ammonium enhanced the number of embryos formed per culture. Accumulation of plumbagin, an important naphthoquinone and a medicinal compound, was three times higher in embryogenic compared to non-embryogenic suspensions.

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Authors and Affiliations

  1. Department of Genetics, Osmania University, 500 007, Hyderabad, India

    P. Komaraiah, C. Jogeswar & P. B. Kavi Kishor

  2. Industrial Biotechnology Division, Reliance Life Sciences Pvt. Ltd., 400 004, Mumbai, India

    S. V. Ramakrishna

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  1. P. Komaraiah
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  2. C. Jogeswar
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  3. S. V. Ramakrishna
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  4. P. B. Kavi Kishor
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Correspondence to P. B. Kavi Kishor.

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Komaraiah, P., Jogeswar, C., Ramakrishna, S.V. et al. Acetylsalicylic acid and ammonium-induced somatic embryogenesis and enhanced plumbagin production in suspension cultures of Plumbago rosea L.. In Vitro Cell.Dev.Biol.-Plant 40, 230–234 (2004). https://doi.org/10.1079/IVP2003502

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  • DOI: https://doi.org/10.1079/IVP2003502

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