Abstract
Acacia mangium microshoots from juvenile and mature genotypes were micropropagated through a regular subculture regime for more than 3 yr in vitro. Average multiplication rates of 5.5 for the juvenile source and of 3.9 for the mature clone were obtained during this period on the 6-benzylaminopurine-enriched multiplication medium. Although the juvenile material displayed higher potential for axillary shoot and root formation than the mature clone overall, the differences were not statistically significant with noticeable variations in the course of time from one subculture to another. On specific rooting media, the juvenile material rooted overall in greater proportions than the mature material, notwithstanding noteworthy interactions between the age of the plant material and the various experimental factors tested, i.e. sucrose concentration, macrosalt formulation and light regime. The stimulating effect of darkness on juvenile plant material rooting rates was more obvious than for the mature clone, which responded more inconsistently. Addition of 4 μM indole-3-acetic acid, indole-3-butyrie acid, or 1-naphthaleneacetic acid in the rooting medium significantly increased the proportion of rooted microshoots of both origins. The rooting criteria observed were also prone to vary depending on the experimental date. The data indicate that rooting of juvenile and mature Acacia mangium materials have average rates of 90% and 77%, respectively. These are high enough to consides possible applications of these procedures toward operational activities.
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Ballester, A.; San-José, M. C.; Vidal, N.; Fernandez-Lorenzo, J. L.; Vieitez, A. M. Anatomical and biochemical events during in vitro rooting of microcuttings from juvenile and mature phases of chestnut. Ann. Bot. 83:619–629; 1999.
Beck, S. L.; Dunlop, R. W. Micropropagation of the Acacia species—a review. In Vitro Cell. Dev. Biol. Plant 37;531–538; 2001.
Bon, M. C.; Bonal, D.; Goh, D. K. S.; Montenuis, O. Influence of diffevent macronutrient solutions and growth regulators on micropropagation of juvenile Acacia mangium and Paraserianthes falcataria explants. Plant Cell Tiss. Organ Cult. 53:171–177; 1998.
Bonga, J. M.; von Aderkas, P. Bejuvenation of tissues from mature conifers and its implications for propagation in vitro. In: Ahuja, M. R.; Libby, W. J., eds. Clonal forestry I: genetics and biotechnology. Berlin and Heidelberg: Springer-Verlag; 1993:182–199.
Champagnat, P.; Barnola, P.; Lavarenne, S. Quelques modalités de la croissance rythmique endogéne des tiges chez les végétaux ligneux. Proccedings of the International Symposium of ‘L'Arbre’. Montpellier, September 9–14, 1985. France: Naturalia Monspeliensia; 1986: 279–302.
Darus, H. A. Vegetative propagation. In: Kamis Awang; Taylor, D., eds. Acacia mangium growing and utilization. MPTS monograph series no. 3. Bungkok: Winrock International and FAO; 1993:59–74.
Galiana, A.; Tibok, A.; Duhoux, E. In vitro propagation of the nitrogen-fixing tree-legume Acacia mangium Wild. Plant. Soil 135:151–159; 1991.
Georse, E. F. Plant propagation by tissue culture, parst 1 and 2. Basingstoke: Exegtics Itd.; 1993.
Hackett, W. P. Phase change and intra-clonal variability. HortScience 18(6):12–16; 1983.
Hackett, W. P. Donor plant maturation and adventitious root formation. In: Davis, T. M.; Haissig, B. E.; Sankhla, N., eds. Adventitious root formation in cuttings. Portland, OR: Dioscorides Press: 1988:11–28.
Haffner, V.; Enjalric, F.; Lardet, L.; Carron, M. P. Maturation of woody plants: a review of metabolic and genomic aspects. Ann. Sci. For. 48:615–630; 1991.
Haissig, B. E.; Riemenschneider, D. E. Genetic effects on adventitious rooting. In: Davis, T. M.; Haissig, B. E.; Sankhla, N., eds. Adventitious root formation in cuttings. Portland, OR: Dioscorides Press: 1988:47–60.
Kamis Awang; Taylor, D. Acacia mangium: growing and utilization. MPTS monograph series no. 3. Bangkok: Winrock Interantional and FAO; 1993.
Mok, M. C.; Martin, R. C.; Mok, D. W. Cytokinins: biosynthesis, metabolism and perception. In Vitro Cell. Dev. Biol. Plant 36:102–107; 2000.
Montenuis, O. Aspects du clonage de séquoias génats (Setmuoiadendron giganteum Buchholz) jcunes et âgés. PhD dissertation. Blaise Pascal University, Clermont Ferrand, France; 1988.
Monteuuis, O.; Bon, M. C. Microbouturage du séquoia géant. Ann. AFOCEL 1985:49–87; 1986.
Montcuuis, O.; Bon, M. C.; Influence of auxins and darkness on in vitro rooting of micropropagated shoots from mature and juvenile Acacia mangium. Plant Cell Tiss. Organ Cult 63:173–177; 2000.
Monteuuis, O.; Bon, M. C.; Berthon, J. Y. Micropropagation aspects of Sequoiadendrom giganteum juvenile and mature clones. Acta Hort. 212:489–497; 1987.
Monteuuis, O.; Vallauri, D.; Poupard, C.; Chauviève, M. Rooting Acacia mangium cuttings of different physiological age with reference to leaf morphology as a phase change marker. Silvae Genet. 44(2/3):150–154; 1995.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Poupard, C.; Chauvière, M.; Monteuuis, O. Rooting Acacia mangium cuttings: effects of age, within-shoot position and auxin treatment. Silvae Genet, 43(4):226–231; 1994.
SAS Institute Inc. SAS/STAT user's guide. Lary, NC: SAS Institute, Inc.; 2000.
Schenk, R. U.; Hildebrandt, A. C. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50:199–204; 1972.
Sokal, R. R.; Rohlf, F. J. Biometry. New York: W. H. Freeman and Co.; 1995.
Thorpe, T. A.; Biondi, S. Conifers In: Sharp, W. R.; Evans, D. A.; Amminato, P. V.; Yamada, Y., eds. Handbook of plant cell culture, vol. 2. New York: Macmillan Publishing Co.; 1934:435–470.
Tran Thanh Van, K. Control of morphogencsis in in vitro cultures. Annu. Bev. Plant. Physiol. 32:291–311; 1931.
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Monteuuis, O. In vitro micropropagation and rooting of Acacia mangium microshoots from juvenile and mature origins. In Vitro Cell.Dev.Biol.-Plant 40, 102–107 (2004). https://doi.org/10.1079/IVP2003493
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DOI: https://doi.org/10.1079/IVP2003493