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Organogenesis and somatic embryogenesis in Ariocarpus kotschoubeyanus (Lem.) K. Schum. (Cactaceae), an endemic and endangered Mexican species

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Summary

Shoots by direct and indirect organogenesis and somatic embryos were induced from tubercles excised from Ariocarpus kotschoubeyanus seeds germinated in vitro. Shoot formation was greatest (6.3 per explant) when explants were cultured on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BA; 13.3 μM) and α-naphthaleneacetic acid (NAA; 5.4 μM). Individualized shoots were rooted in half-strength MS; the addition of activated charcoal (1 g l−1) and the use of sun cap closures to seal containers improved the rooting of shoots. Nearly 20% of the explants produced somatic embryos on media containing combinations of BA (8.9–22.2 μM) and NAA (0.5–5.4 μM). The establishment of plantlets in soil presented no significant problems. In vitro culture is a useful option for mass propagation of A. kotschoubeyanus and contributes to its conservation.

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References

  • Anderson, E. F. A taxonomic revision of Ariocarpus (Cactaceae). Cact. Succ. J. (US) 37:39–49; 1965.

    Google Scholar 

  • Anderson, E. F.; Arias, S.; Taylor, N. P.; Cattabriga, A. Threatened cacti of Mexico. London: Royal Botanical Gardens, Kew; 1994.

    Google Scholar 

  • Anonymous. Norma Oficial Mexicana NOM-ECOL-059-1994, que determina las especies de flora y fauna silvestres terrestres y acuáticas en peligro de extinción, amenazadas, raras y las sujetas a protección especial, y que establece especificaciones para su protección. Diario Oficial de la Federación; May 16, 1994:13–14.

  • Arias, M. S. Cactáceas: conservación y diversidad en México. Rev. Soc. Mex. Hist. Nat. Vol. Esp. 44:109–115; 1993.

    Google Scholar 

  • Clayton, P. W.; Hubstenberger, J. F.; Phillips, G. C. Micropropagation of members of the Cactaceae subtribe Cactinae. J. Am. Soc. Hort. Sci. 115:337–343; 1990.

    CAS  Google Scholar 

  • Corona, N. V.; Yañez, L. L. Propagación de Cephalocereus senilis mediante cultivo de tejidos. Cact. Suc. Mex. 29:3–7; 1984.

    Google Scholar 

  • Dabekaussen, M. A. A.; Pierik, R. L. M.; Van der Laken, J. D.; Spaans, J. H. Factors affecting areole activation in vitro in the cactus Sulcorebutia alba Rausch. Scientia Hort. 46:283–294; 1991.

    Article  CAS  Google Scholar 

  • Debergh, P. Improving micropropagation. IAPTC Newsletter (51):2–10; 1989.

    Google Scholar 

  • Fay, M. F. In what situations is in vitro culture appropriate to plant conservation? Biodiversity Conserv. 3:176–183; 1994.

    Article  Google Scholar 

  • Fay, M. F.; Gratton, J. Tissue culture of cacti and other succulents: a literature review and a report on micropropagation at Kew. Bradleya 10:33–48; 1992.

    Google Scholar 

  • George, E. F. Plant propagation by tissue culture. London: Exegetics Ltd; 1993.

    Google Scholar 

  • Gray, D. J.; Purohit, A. Somatic embryogenesis and development of synthetic seed technology. Crit. Rev. Plant Sci. 10:33–61; 1991.

    Article  Google Scholar 

  • Hubstenberger, J. F.; Clayton, P. W.; Phillips, G. C. Micropropagation of cacti (Cactaceae). In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry vol. 20. High-tech and micropropagation IV. Berlin, Heidelberg: Springer-Verlag; 1992:49–68.

    Google Scholar 

  • Hunt, D. CITTES: Cactaceae checklist. London: Royal Botanic Gardens, Kew; 1992.

    Google Scholar 

  • Infante, R. In vitro axillary shoot proliferation and somatic embryogenesis of yellow pitaya Mediocactus coccineus (Slam-Dyck). Plant Cell Tiss. Organ Cult. 31:155–159; 1992.

    Article  CAS  Google Scholar 

  • Johnson, J. L.; Emino, E. R. Tissue culture propagation in the Cactaceae. Cact. Succ. J. (US) 51:275–277; 1979.

    Google Scholar 

  • Krulik, C. Tissue culture of succulent plants. Nat. Cact. Succ. J. 35(1):14–17; 1980.

    Google Scholar 

  • Marín, H. T. Inducción de Respuestas Morfogenéticas in vitro en Mammillaria san-angelensis especie en peligro de extinción. Tesis Doctoral (Ciencias), Fac. Ciencias, U.N.A.M. México, D. F.; 1998.

    Google Scholar 

  • Martínez-Vázquez, O.; Rubluo, A. In vitro mass propagation of the nearextinet Mammillaria san-angelensis Sánchez-Mejorada. J. Hort. Sci. 99–105; 1989.

  • Mata, R. M.; Monroy, R. M. A.; Moebius, G. K.; Chávez, A. V. M. Micropropagation of Turbinicarpus laui Glass et Foster, an endemic and endangered species. In Vitro Cell. Dev. Biol. Plant 37:400–404; 2001.

    Article  Google Scholar 

  • Mauseth, J. D. A new method for the propagation of cacti: sterile culture of axillary buds. Cact. Succ. J. (US) 51:186–187; 1979.

    Google Scholar 

  • Minocha, S. C.; Mehra, P. N. Nutritional and morphogenetic investigations on callus cultures of Neomammillaria prolifera Miller (Cactaceae). Am. J. Bot. 6:168–173; 1974.

    Article  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Oldfield, S. Cactus and succulent plants—status survey and conservation action plan. IUCN/SSC Cactus and Succulent Specialist Group. Gland and Cambridge: IUCN; 1997.

    Google Scholar 

  • Olguín, L. P. Cultivo in vitro de Ariocarpus retusus Scheidw. (Cactaceae) especie en peligro de extinción. Tesis de Licenciatura (Biólogo), Facultad de Ciencias, U.N.A.M. México, D.F.; 1994.

    Google Scholar 

  • Ortiz-Montiel, J. G.; Alcántara, G. R. Propagación in vitro de Peyote (Lophophora williamsii (Lemaire) Coulter). Cact. Suc. Mex. 42(1):3–6; 1997.

    Google Scholar 

  • Pérez, E. M. B.; Pérez, M. E.; Villalobos, E.; meza, E.; Morones, L. R.; Lizalde, H. J. Micropropagation of 21 species of Mexican cacti by axillary proliferation. In Vitro Cell. Dev. Biol. Plant 34:131–135; 1998.

    Google Scholar 

  • Rabenda, I. Breaking dormancy in cactus seed. Part 1. Cact. Suce. J. (US) 62:86–94; 1990.

    Google Scholar 

  • Reyes, S. J. Propagación de Cactáceas Mexicanas: una alternativa para la conservación de especies amenazadas y en peligro de extinción. In: Encuentro Internacional sobre el Impacto de la Biotecnología en el Desarrollo Sustentable. PROMESUP, OEA; 1994:108–119.

  • Rodríguez-Garay, B.; Rubluo, A. In vitro morphogenetic responses of the endangered cactus Aztekium ritteri (Boedeker). Cact. Succ. J. (US) 64:116–119; 1992.

    Google Scholar 

  • Starling, R. In vitro propagation of Leuchtenbergia principis. Cact. Succ. J. (US) 57:114–115; 1985.

    Google Scholar 

  • Starling, R. J.; Hutson, R. Sterile culture of succulent plants. Br. Cact. Succ. J. 2(3):69–70; 1984.

    Google Scholar 

  • Stuppy, W.; Nagl, W. Regeneration and propagation of Ariocarpus retusus Scheidw. (Cactaceae) via somatic embryogenesis. Bradleya 10:85–88; 1992.

    Google Scholar 

  • Torrez-Muñoz, L.; Rodríguez-Garay, B. Somatic embryogenesis in the threatened cactus Turbinicarpus pseudomacrochele (Buxbaum & Backeberg). J. Prof. Assoc. Cactus Dev. 1:36–38; 1996.

    Google Scholar 

  • Vovides, A. P.; Luna, V.; Medina, G. Relación de algunas plantas y hongos mexicanos raros, amenazados o en peligro de extinción y sugerencias para su conservación. Acta Bot. Mexicana 39:1–42; 1997.

    Google Scholar 

  • Vyskot, B.; Jara, Z. Clonal propagation of cacti through axillary buds in vitro. J. Hort. Sci. 59:449–452; 1984.

    Google Scholar 

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Authors and Affiliations

  1. Jardín Botánico del Instituto de Biología, Universidad Nacional Autónoma de México, 04510, D.F., Mexico

    Katja G. Moebius-Goldammer & Victor M. Chávez-Avila

  2. Laboratorio de Cultivo de Tejidos, Jardín Botánico Francisco Javier Clavijero, Instituto de Ecología, A.C., Xalapa, Apdo. Postal 63, 91070, Veracruz, Mexico

    Martín Mata-Rosas

Authors
  1. Katja G. Moebius-Goldammer
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  2. Martín Mata-Rosas
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  3. Victor M. Chávez-Avila
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Correspondence to Martín Mata-Rosas.

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Moebius-Goldammer, K.G., Mata-Rosas, M. & Chávez-Avila, V.M. Organogenesis and somatic embryogenesis in Ariocarpus kotschoubeyanus (Lem.) K. Schum. (Cactaceae), an endemic and endangered Mexican species. In Vitro Cell.Dev.Biol.-Plant 39, 388–393 (2003). https://doi.org/10.1079/IVP2003427

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  • DOI: https://doi.org/10.1079/IVP2003427

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