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In vitro axillary shoot proliferation and somatic embryogenesis of yellow pitaya Mediocactus coccineus (Salm-Dyck)

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Abstract

Yellow pitaya (Mediocactus coccineus) seeds were sown on Murashige and Skoog (1962) mineral salt medium. After germination, epicotyls were placed on media enriched with a combination of naphthaleneacetic acid (NAA) (0.05, 0.27 or 0.54 μM) and benzyladenine (BA) (2.2 or 4.4 μM). The apical tip was excised from half of the shoots and the other half were kept intact. Different values for proliferation rate, shoot length and thickness were observed on each medium. The cotyledons and roots were placed on MS medium supplemented with NAA (2.7 or 5.4 μM) and embryogenic calluses were formed. Somatic embryos were induced on these media and then they normally developed on a growth regulator-free medium.

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Abbreviations

BA:

benzyladenine

MS:

Murashige and Skoog

NAA:

α-naphthalenacetic acid

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Author notes

  1. Rodrigo Infante

    Present address: Istituto di Coltivazioni Arboree, Università di Bologna, V. Filippo Re 6, 40126, Bologna, Italy

Authors and Affiliations

  1. Escuela de Agronomía, Universidad de Talca, Casilla 747, Talca, Chile

    Rodrigo Infante

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  1. Rodrigo Infante
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Infante, R. In vitro axillary shoot proliferation and somatic embryogenesis of yellow pitaya Mediocactus coccineus (Salm-Dyck). Plant Cell Tiss Organ Cult 31, 155–159 (1992). https://doi.org/10.1007/BF00037700

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  • DOI: https://doi.org/10.1007/BF00037700

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