Summary
A method has been developed for the induction of adventitious shoots from leaf tissue of Echinacea pallida with subsequent whole-plant regeneration. Proliferating callus and shoot cultures were derived from leaf tissue explants placed on Murashige and Skoog medium supplemented with 6-benzylaminopurine and naphthaleneacetic acid combinations. The optimum shoot regeneration frequency (63%) and number of shoots per explant (2.3 shoots per explant) was achieved using media supplemented with 26.6 μM 6-benzylaminopurine and 0.11 μM naphthaleneacetic acid. Rooting of regenerated shoot explants was successful on Murashige and Skoog medium, both with and without the addition of indole-3-butyric acid. All plantlets survived acclimatization, producing phenotypically normal plants in the greenhouse. This study demonstrates that leaf tissue of E. pallida is competent for adventitious shoot regeneration and establishes a useful method for the micropropagation of this important medicinal plant.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barret, B.; Vohmann, M.; Calabrese, C. Echinacea for upper respiratory infection. J. Fam. Pract. 48:628–635; 1999.
Bauer, R.; Wagner, H. Echinacea species as potential immunostimulatory drugs. In: Wagner, H.; Farnsworth, N. R., eds. Economic and medicinal plant research, vol. 5. London: Academic Press; 1991:253–321.
Choffe, K.; Murch, S.; Saxena, P. Regeneration of Echinacea purpurea: induction of root organogenesis from hypocotyls and cotyledon explants. Plant Cell Tiss. Org. 63:227–234; 2001.
Choffe, K.; Victor, J.; Murch, S.; Saxena, P. In vitro regeneration of Echinacea purpurea L.: direct somatic embryogenesis and indirect shoot organogenesis in petiole culture. In Vitro Cell. Dev. Biol. Plant 36:30–36; 2000.
Harbage, J. F. Micropropagation of Echinacea angustifolia, E. pallida, and E. purpurea from stem and seed explants. HortScience 36:360–364; 2001.
Hu, C.; Kitts, D. D. Studies on the antioxidant activity of Echinacea root extract. J. Agric. Food Chem. 48:1466–1472; 2000.
Koroch, A.; Juliani, H. R.; Kapteyn, J.; Simon, J. E. In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tiss. Organ Cult. 69:79–83; 2002.
Lakshmanan, P.; Danesh, M.; Taji, A. Production of four commercially cultivated Echinacea species by different methods of in vitro regeneration. J. Hort. Sci. Biotechnol. 77:158–163; 2002.
McGregor, R. L. The taxonomy of the genus Echinacea (Compositae). Univ. Kansas Sci. Bull. 48:113–142; 1968.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Percival, S. S. Use of Echinacea in medicine. Biochem. Pharmacol. 60:155–158; 2000.
Sari, A. O.; Morales, M. R.; Simon, J. E. Ethephon can overcome seed dormancy and improve seed germination in purple coneflower species Echinacea angustifolia and E. pallida. HortTechnology 11:202–205; 2001.
Speroni, E.; Govini, P.; Guizzardi, S.; Renzulli, C.; Guerra, M. C. Antiinflammatory and cicatrizing activity of Echinacea pallida Nutt. root extract. J. Ethnopharmacol. 79:265–272; 2002.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Koroch, A.R., Kapteyn, J., Juliani, H.R. et al. In vitro regeneration of Echinacea pallida from leaf explants. In Vitro Cell.Dev.Biol.-Plant 39, 415–418 (2003). https://doi.org/10.1079/IVP2003424
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1079/IVP2003424