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In vitro propagation of Acacia sinuata (Lour.) Merr. from nodal segments of a 10-year-old tree

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Summary

An in vitro propagation protocol has been developed using nodal explants from a mature ‘elite’ tree of Acacia sinuata. Tissue browning was circumvented by soaking surface-disinfected explants in a solution of antioxidant (238 μM citric acid). Maximum shoot proliferation (75.2%) was achieved from nodal explants collected during the December to March season in Murashige and Skoog's (MS) medium supplemented with 8.9μM 6-benzyladenine (BA), 2.5μM thidiazuron (TDZ), and 135.7μM adenine sulfate (AS) at the end of the first transfer following initial culture (60 d after inoculation). Gibberellic acid (GA3) at 1.8 μM promoted shoot elongation. The number of shoots was increased by (1) repeated subculturing of nodal explants on fresh medium with the same composition, and (2) using microcuttings from in vitro-regenerated shoots on MS medium containing 6.6 μM BA where each node produced four shoots. When transferred to half-strength MS medium augmented with 7.4 μM indolebutyric acid (IBA) in vitro-regenerated shoots produced prominent roots. Rooted plants were hardened and successfully established in soil. This protocol yielded an average of 100 plants per nodal explant over a period of 3 mo.

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Authors and Affiliations

  1. Department of Biotechnology, School of Life Sciences, Bharathidasan University, 620 024, Tiruchirappalli, Tamilnadu, India

    G. Vengadesan, A. Ganapathi, R. Pream Anand & N. Selvaraj

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  1. G. Vengadesan
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  2. A. Ganapathi
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  3. R. Pream Anand
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  4. N. Selvaraj
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Correspondence to G. Vengadesan.

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Vengadesan, G., Ganapathi, A., Anand, R.P. et al. In vitro propagation of Acacia sinuata (Lour.) Merr. from nodal segments of a 10-year-old tree. In Vitro Cell.Dev.Biol.-Plant 39, 409–414 (2003). https://doi.org/10.1079/IVP2003421

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