Summary
An efficient protocol has been developed for the regeneration of plantlets from leaf explants of witloof chicory (Cichorium intybus L.). Regeneration via callus was obtained on modified Murashige and Skoog semisolid medium (MS) containing 2.0 μM indole-3-acetic acid +5.0 μM 6-furfurylaminopurine (kinetin), and 1000 mgl−1 casein hydrolyzate. At least five or more shoots regenerated from each callus. The shoots were rooted on MS +0.2 μM indole-3-butyric acid. The plantlets thus obtained were successfully established in soil after bardening. Esculin accumulation was recorded in plant tissues at different stages of differentiation in in vitro cultures and compared with in vivo-grown, plants. The esculin accumulation was higher in in vitro plants.
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Rehman, R.U., Israr, M., Srivastava, P.S. et al. In vitro regeneration of witloof chicory (Cichorium intybus L.) from leaf explants and accumulation of esculin. In Vitro Cell.Dev.Biol.-Plant 39, 142–146 (2003). https://doi.org/10.1079/IVP2002381
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DOI: https://doi.org/10.1079/IVP2002381