Summary
A method for the micropropagation of Zantedeschia albomaculata is presented using shoot tip proliferation onto Murashige and Skoog (MS) medium supplemented with different plant growth regulator concentrations and combinations. Of the four cytokinins tested, 6-benzyladenine (BA) and thidiazuron (TDZ) were found to be more effective. An optimal concentration of BA (8.87 μM) or TDZ (4.54 μM) developed an average of 3.8 and 3.2 shoots per explant, respectively, but increasing concentrations of cytokinins often led to lower proliferation rate and stunted growth. Addition of auxins to the MS medium supplemented with 8.87 μM BA slightly enhanced multiple shoot formation in the explants. Multiplication of six cultivars of Zantedeschia genus comprising different flower types and colors were tested and achieved using only one regeneration medium (MS+8.87 μMBA+2.46 μM IBA). Different MS medium strength, air temperature (15, 20, 25, and 30°C) and light quality [fluorescent, red + blue, red and blue light provided by a LED (light-emitting diode) system] were used (without phytohormone) with the aim of stimulating in vitro shoot and root development. Half-strength MS or MS and cultures maintained at 25°C were found to be equally suitable for shoot tip culture of Z. albomaculata. Shoot elongation as well as fresh and dry weight were significantly increased when cultures were kept under red or blue light.
Similar content being viewed by others
References
Ahroni, A.; Zukur, A.; Rozen, Y.; Shejtman, H.; Vain Stein, A. An efficient method for adventious shoot regeneration from stem segment explants of gypsophila. Plant Cell Tiss. Organ Cult. 49:101–106; 1997.
Akoyunoglou, G.; Anni, H. Blue light effect on chloroplast development in higher plants. In: Senger, H., ed. Blue light effects in biological systems. Berlin: Springer-Verlag; 1984:397–406.
Appelgren, M. Effects of light quality on stem elongation of Pelargonium in vitro. Sci. Hortic. 45:345–351; 1991.
Ballare, C. L.; Scopel, A. L.; Sanchez, R. A. Photocontrol of stem elongation in plant neighborhoods: effects of photon fluence rate under natural conditions of radiation. Plant Cell Environ. 14:57–65; 1991.
Brown, C. S.; Schuerger, A. C.; Sager, J. C. Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J. Am. Soc. Hort. Sci. 120:808–813; 1995.
Bruyn, M. H. D.; Ferreira, D. I.; Slabbert, M. M.: Pretorius, J. In vitro propagation of Amryllis belladonna. Plant Cell Tiss. Organ Cult. 31:179–184; 1992.
Bula, R. J.; Morrow, R. C.; Tibbitts, T. W.; Ignatius, R. W.; Martin, T. S.; Barta, D. J. Light emitting diodes as a radiation source for plants. HortScience 26:203–205; 1991.
Chakrabarty, D.; Mandal, A. K. A.; Datta, S. K. Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.). J. Hort. Sci. Biotechnol. 74:293–296, 1999.
Chen, J. J.; Liu, M. C.; Ho, Y. H. Size of in vitro plantlets affects subsequent tuber production of acclimated calla lily. HortScience 35:290–292; 2000.
Chen, Y. Q.; Piluex, C. Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis. Plant Growth Reg. 16:99–101; 1995.
Clemens, J.; Welsh, T. E.; An overview of the New Zealand calla industry. research direction and year round tuber production. Acta Hort. 337:161–166; 1993.
Cohen, D. Micropropagation of Zantedeschia hybrids. Comb. Proc. Int. Plant Prop. Soc. 31:312–316; 1981.
Economou, A. S.; Read, P. E. Light treatments to improve efficiency of in vitro propagation systems. HortScience 22:751–754; 1937.
Fang, W. L.; Xiong, L.; Qu, Y. H.; Qu, S. P. Tissue culture of colored common calla lily. J. South West Agri. Univ. 21:423–426; 1999.
Hanson, H. C. Leal structure as related to environment. Am. J. Bot. 4:533–560; 1917.
Höel, B. O.; Solihang, K. A. Effect of irradiance on chlorophyll estimation with the Minolta SPAD-502 leaf chlorophyll meter. Ann. Bot. 82:389–392; 1998.
Huetteman, C. A.; Preece, J. E. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Organ Cult. 33:105–119; 1993.
Kritzinger, E. M.; Vuuren, R. J. V.; Woodward, B.; Rong, I. H.; Spreeth, M. H.; Slabbert, M. M. Elimination of external and internal contaminants in rhizomes of Zantedeschia aethiopica with commercial fungicides and antibiotics. Plant Cell Tiss. Organ Cult. 52:61–65; 1998.
Kuehny, J. S. Calla history and culture. Hort. Technol. 10:267–274; 2000.
Lian, M. L.; Murthy, H. N.; Paek, K. Y. Effects of light emitting diodes on the in vitro induction and growth of bulblets of Lilium oriental hybrid ‘Pesaro’. Sci. Hortic. 94:365–370; 2002.
Morgan, D. C.; Smith, H. Non-photosynthetic responses to light quality. In: Pirson, A.; Zimmerman, M. H., eds. Encyclopedia of plant physiology. New series 12A. Berlin: Springer-Verlag: 1981: 109–134.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Robin, C.; Hay, M. J. M.; Newton, P. C. D.; Greer, D. Effect of light quality (red: far-red ratio) at the apical bud of the main stolon on morphogenesis of Trifolium repens L. Ann. Bot. 72:119–123; 1994.
Ruiz, S. G.; Rosa, M. E.; Flores, O. C. E. Zantedeschia aethiopica propagation by tissue culture. J. Agri. Uni. Puerto-Rico. 80:193–194; 1996.
Saebo, A.; Krekling, T.; Appelgren, M. Light quality affects photosynthesis and leaf anatomy of brich plantlets in vitro. Plant Cell Tiss. Organ Cult. 41:177–185; 1995.
Seabrook, J. E. A.; Cumming, B. G.; Dionne, L. A. The in vitro induction of adventitious shoot and root apices on Narcissus cultivars tissue. Can. J. Bot. 54:813–819; 1976.
Senger, H. The effect of blue light on plants and microorganisms. Phytochem Photobiol. 35:911–920; 1982.
Sims, D. A.; Pearcy, R. W. Response of leaf anatomy and photosynthetic capacity in Alocasia macrorrhiza (Araceae) to a transfer from low to high light. Am. J. Bot. 79:449–455; 1992.
Sriskandarjah, S.; Skirvin, R. M.; Abu-Qaoud, H. The effect of some macronutrients on adventitious root development on scion apple cultivars in vitro. Plant Cell Tiss. Organ Cult. 21:185–189; 1990.
Tanaka, M.; Takamura, T.; Watanabe, H.; Endo, M.; Yanagi, T.; Okamoto, K. In vitro growth of Cymbidium plantlets cultured under superbright red and blue light-emitting diodes (LEDs). J. Hort. Sci. Biotechnol. 73;39–44; 1998.
Tennessen, D. J.; Singsaas, E. L.; Sharkey, T. D. Light emitting diodes as a light source for photosynthesis research. Photosyn. Res. 39:85–92; 1994.
Tjia, B. Zantedeschia. In: Halevy, A. H., ed. Handbook of flowering. Boca Raton, FL: CRC Press; 1989:697–702.
Welsh, T. E.; Clemens, J. Protected cropping of Zantedeschia tubers and cutflowers in New Zealand. Acta Hort. 319:335–340; 1992.
Zuker, A.; Ahroni, A.; Shejtman, H.; Vain Stein, A. Adventitious shoot generation from leaf explants of Gypsophila paniculata L. Plant Cell Rep. 16:775–778; 1997.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chang, H.S., Charkabarty, D., Hahn, E.J. et al. Micropropagation of calla lily (Zantedeschia albomaculata) via in vitro shoot tip proliferation. In Vitro Cell.Dev.Biol.-Plant 39, 129–134 (2003). https://doi.org/10.1079/IVP2002362
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1079/IVP2002362