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In vitro nitrogen nutrition and hormonal pattern in bromeliads

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Summary

Four cytokinins (CKs), indole-3-acetic acid (IAA) and its ester and amino conjugates, and abscisic acid (ABA) levels of two bromeliads, Ananas comosus (L.) Merril and Vriesea gigantea Gaudich., grown in 5 mM (NH4)2SO4 or urea as the sole nitrogen (N) form, were investigated. In both bromeliads, zeatin (Z) and zeatin riboside ([9R]Z) were the most abundant CKs. In A. comosus, CKs levels decreased drastically (≊ 12 times) after 7 and 30 d in media with ammonium and urea, respectively. After 3 d in media with N, V. gigantea CK levels decreased 30 and 20 times in the presence of ammonium and urea, respectively. N-starved A. comosus and V. gigantea exhibited similar ABA levels, but ABA decreased faster in V. gigantea when plants were transferred to media with N. Free IAA levels decreased until the 15th and 30th day when A. comosus was transferred to a medium with ammonium and urea, respectively. N-starved A. comosus amide, ester, and free IAA amounted to 81%, 14%, and 4%, respectively. There was a transient increase in the proportion of amide IAA and a corresponding decrease of the ester and the free IAA proportion when N-starved plants were transferred to media with N. The relationship between the internal hormonal patterns and the different ecological adaptations of the two bromeliads are discussed.

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  1. Lauricio Endres

    Present address: Depto Botânica, CCBi, UFAL, Praça Afrânio Jorge S/N, Bairro Prado, CEP 57011-020, Maceió, AL, Brazil

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  1. Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, C.P. 11461, CEP 05422-970, São Paulo, SP, Brazil

    Lauricio Endres, Beatriz Maia Souza & Helenice Mercier

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  2. Beatriz Maia Souza
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Endres, L., Souza, B.M. & Mercier, H. In vitro nitrogen nutrition and hormonal pattern in bromeliads. In Vitro Cell Dev Biol -Plant 38, 481–486 (2002). https://doi.org/10.1079/IVP2002323

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