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Shoot development in Quercus suber L. somatic embryos

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Summary

N6-Benzyladenine (BA; 0.04–4μM) application to germinated Quercus suber somatic embryos considerably increased caulinar apex elongation frequency and maintained active growth in the plantlets, although it did not have a significant effect on the percentage of shoots with normal morphology. The addition of 0.5 μM indoleacetic acid together with the cytokinin did not have any effect. The use of a low concentration (0.04 μM) of BA allowed the appropriate radicle elongation in all germinating somatic embryos, but higher concentrations arrested this elongation.

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Author notes

  1. Gemma García-Martín

    Present address: Dirección General de Investigación, Consejería de Educación, Comunidad de Madrid, C/Alcalá 32, 3a planta, 28014, Madrid, Spain

  2. José Antonio Manzanera

    Present address: Instituto Madrileño de Investigación Agraria, Autovía de Aragón Km.36, Aptdo. Correos 127, Alcalá de Henares, 28800, Madrid, Spain

Authors and Affiliations

  1. Departamento de Biología Vegetal, Escuela Universitaria de Ingeniería Técnica Agrícola, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040, Madrid, Spain

    M. Elena González-Benito

  2. Instituto Madrileño de Investigación Agraria, Autovía de Aragón Km.36, Aptdo. Correos 127, Alcalá de Henares, 28800, Madrid, Spain

    Gemma García-Martín

  3. Departamento de Silvopascicultura, Universidad Politécnica de Madrid, Ciudad Universitaria, 28040, Madrid, Spain

    José Antonio Manzanera

Authors
  1. M. Elena González-Benito
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  2. Gemma García-Martín
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  3. José Antonio Manzanera
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Correspondence to M. Elena González-Benito.

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González-Benito, M.E., García-Martín, G. & Antonio Manzanera, J. Shoot development in Quercus suber L. somatic embryos. In Vitro Cell Dev Biol -Plant 38, 477–480 (2002). https://doi.org/10.1079/IVP2002318

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  • DOI: https://doi.org/10.1079/IVP2002318

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