Summary
A procedure for micropropagation of pitaya using thidiazuron (TDZ) and naphthaleneacetic acid (NAA) is described. Explants were excised from young joints of mature plants and cultured on Murashige and Skoog medium (MS) containing 0.5 μM NAA and 0.5 μM TDZ. Shoots produced from these first explants were cut up to produce secondary explants, either by decapitation or by longitudinal division into three parts. The decapitated and longitudinal explants were cultured on MS supplemented with 0.5 μM NAA and either 0.01, 0.09, 0.5, or 0.9 μM TDZ. Decapitated explants produced more shoots at higher frequency that the longitudinal explants. For both types of secondary explants, most shoots were developed from the distal parts. Shoots produced from secondary explants were rooted in MS and then transferred to soil where they produced normal plants.
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Barbeu, G. The strawberry pear, a new tropical fruit. Fruits 45: 141–147; 1990.
Ebert, G.; Fischer, G.; Ludders, P. Pitaya—a new fruit species from Colombia. Erwerbsobstbau. 35:49–52; 1993.
Fouque, A. Especes fruitieres d'Amerique tropicale. Fruits 27:200–218; 1972.
Infante, R. In vitro axillary shoot proliferation and somatic embryogenesis of yellow pitaya Mediocactus coccineus (Salm-Dyck). Plant Cell Tiss. Organ Cult. 31:155–159; 1992.
Johnson, J. L.; Emino, E. R. Tissue culture propagation in the cactaceae. Cact. Succ. J. 51:275–277; 1979.
Jorge, L. F.; Ferro, V. O. Anatomical and phytochemical aspects of Hylocereus undatus (Haworth) Britton & Rose. Revista Farmacia Bioquimica Univ. Sao Paulo. 25:123–136; 1989.
Martin, F. W.; Campell, C. W. A.; Ruberte, R. M. Perennial edible fruits of the tropics: an inventory. USDA, ARS. Series: Agriculture Handbook USDA 0065-4612, no. 642; 1987.
Mizrahi, Y.; Nerd, A. New crops as a possible solution for the troubled Israeli export market. Wanatca Yearbook 20:41–51; 1996.
Mohamed-Yasseen, Y. Induction of lateral shoot and bud formation from cactus and succulent plants with promaline and thidiazuron. Plant Growth Regul. Soc. Am. 22:27; 1994a.
Mohamed-Yasseen, Y. In vitro flower and plant formation from Opuntia delinii Haw. and Opuntia cochenillifera Mill. Am. Soc. Hort. Sci., Southern Regional Mtngs (Abstr.). HortScience 29:880; 1994b.
Mohamed-Yasseen, Y.; Barringer, S. A.; Splittstoesser, W. E. A note on the uses of Opuntia spp. in central/North America: aspects and future prospects. J. Arid Environ. 32:347–353; 1995a.
Mohamed-Yasseen, Y.; Barringer, S. A.; Splittstoesser, W. E.; Schnell, R. J. Rapid propagation of tuna (Opuntia ficus-indica) and plant establishment in soil. Plant Cell Tiss. Organ Cult. 42:117–119; 1995b.
Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.
Rodrigues, D. J.; Ocampo, H. L.; Casillas, G. J. Extraction of a natural colarant from peel of pitahaya (Hylocerus undatus) fruit. Tecno. Aliment. 30:22–26; 1995.
Shimomura, T.; Fujihara, K. Stimulation of axillary shoot formation of cuttings of Hylocereus trigonus (Cactaceae) by pre-soaking in benzyladenine solution. Sci. Hortic. 13:289–296; 1980.
Wu, M. C.; Chen, S. C. Variation of sugar content in various parts of pitaya fruit. Proc. Fl. State Hortic. Soc. 110:225–227; 1997.
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Mohamed-Yasseen, Y. Micropropagation of pitaya (Hylocereus undatus Britton et rose). In Vitro Cell Dev Biol -Plant 38, 427–429 (2002). https://doi.org/10.1079/IVP2002312
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DOI: https://doi.org/10.1079/IVP2002312