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Photoautotrophic culture conditions and photosynthetic photon flux influence growth of Lilium bulblets in vitro

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Summary

Lilium Asiatic hybrid ‘Mona’ bulblets were cultured in vitro for 100 d under photoautotrophic (CO2-enriched conditions and without sucrose in the medium) and heterotrophic (non-enriched CO2 conditions and sucrose-supplemented medium) methods and under various levels of photosynthetic photon flux (PPF). Bulblet growth and net photosynthetic rate (NPR) were analyzed. CO2− and PPF-enriched conditions enhanced the overall growth of bulblets, scale leaves, and roots. Heterotrophic conditions enhanced bulblet growth but higher PPF levels were inhibitory to the development of scale leaves. These results indicate the CO2− and PPF-enriched conditions (photoautotrophic conditions) are beneficial for the production of high-quality bulblets of Asiatic hybrid lilies in vitro

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References

  • Adelberg, J.; Fujiwara, K.; Kirdmanee, C.; Kozai, T. Photoautotrophic shoot and root development for triploid melon. Plant Cell Tiss. Organ Cult. 57:95–104; 1999.

    Article  Google Scholar 

  • Cui, Y. Y.; Hahn, E. J.; Kozai, T.; Paek, K. Y. Number of air exchanges, sucrose concentration, photosynthetic photon flux and differences in photoperiod and dark period temperatures affect growth of Rehmannia gluticosa plantlets in vitro. Plant Cell Tiss. Organ Cult. 62:219–226; 2000.

    Article  CAS  Google Scholar 

  • Doi, M.; Oda, H.; Asahira, T. In vitro atmosphere of cultured C3 and CAM plants in relation to day length. Environ. Contr. Biol. 27:9–13; 1989.

    Google Scholar 

  • Fujiwara, K.; Kozai, T.; Watanabe, I. Measurements of CO2 concentration in stoppered vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets (in Japanese with English summary). J. Agric. Meterol. 43:21–30; 1987.

    Google Scholar 

  • Fujiwara, K.; Kozai, T.; Watanabe, I. Development of photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimation stages. Acta. Hort. 230:153–158; 1988.

    Google Scholar 

  • Kirdmanee, C.; Kitaya, Y.; Kozai, T. Effects of CO2 enrichment and supporting material in vitro on photoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro. In Vitro Cell. Dev. Biol. Plant 31:144–149; 1995.

    Google Scholar 

  • Kozai, T. Micropropagation under photoautotrophic conditions. In: Deberg, P. C.; Zimmerman, R. H.; eds. Micropropagation: technology and applications. Dordrecht: Kluwer Academic Publishers; 1991:447–469.

    Google Scholar 

  • Kozai, T.; Iwabuchi, K.; Watanabe, K.; Watanabe, I. Photoautotrophic and photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium. Plant Cell Tiss. Organ Cult. 25:107–115; 1991.

    Google Scholar 

  • Kozai, T.; Iwanami, I. Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of carnation (Dianthus caryophyllus L.) in tissue culture during preparation stage. J. Jap. Soc. Hort. Sci. 57:279–288; 1988.

    Google Scholar 

  • Kozai, T.; Koyama, Y.; Watanabe, I. Multiplication and rooting of potato plantlets in vitro with sugar free medium under high photosynthetic photon flux. Acta Hort. 230:121–127; 1988.

    Google Scholar 

  • Kozai, T.; Oki, H.; Fujiwara, K. Photosynthetic characteristics of Cymbidium plantlets in vitro. Plant Cell Tiss. Organ Cult. 22:205–211; 1990.

    Article  Google Scholar 

  • Kubota, C.; Kakizaki, N.; Kozai, T.; Kasahara, K.; Nemoto, J. Growth and net photosynthetic rate of tomato plantlets during photoautotrophic and photomixotrophic micropropagation. HortScience 36:49–52; 2001.

    CAS  Google Scholar 

  • Mousseau, M. CO2 enrichment in vitro. Effects on autotrophic and heterotrophic cultures of Nicotiana tobacum (var. Samsun). Photosynth. Res. 8:187–191; 1986.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Nakayama, M.; Kozai, T.; Watanabe, K. Effect of presence/absence of sugar in the medium and natural/forced ventilation on net photosynthetic rates of potato plantlets in vitro. Plant Tiss. Cult. Lett. 8:105–109; 1991.

    CAS  Google Scholar 

  • Niimi, Y. In vitro propagation and post in vitro establishment of bulblets of Lilium japanicum Thumb. J. Jap. Soc. Hort. Sci. 63:843–852; 1995.

    Article  Google Scholar 

  • Tisserat, B.; Herman, C.; Silman, R.; Bothast, R. J. Using ultra-high carbon dioxide levels enhances plantlet growth in vitro. HortTechnol. 7:282–289; 1997.

    Google Scholar 

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Correspondence to Paek Kee-Yoeup.

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Mei-Lan, L., Murthy, H.N. & Kee-Yoeup, P. Photoautotrophic culture conditions and photosynthetic photon flux influence growth of Lilium bulblets in vitro . In Vitro Cell.Dev.Biol.-Plant 39, 532–535 (2003). https://doi.org/10.1079/IVP2003440

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  • DOI: https://doi.org/10.1079/IVP2003440

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