Abstract
A shake-elution procedure was evaluated for quantifying root lesion nematodes (Pratylenchus thornei) in roots of chickpea (Cicer arietinum) and wheat (Triticum aestivum). The procedure was a combination of previously described techniques: shaking root pieces in water, sieving the water to collect the nematodes and centrifuging the nematode sample over a sucrose cushion to remove root debris. The procedure gave very clean samples of P. thornei in which the nematodes were active and readily identifiable. A statistical (failure time) model was used to describe and compare recovery profiles (cumulative recovery over time). The shake-elution procedure recovered nematodes up to 8.3 times faster than a Baermann procedure and up to 2.4 times faster than a misting procedure. For a given experiment, recovery profiles for chickpea and wheat were very similar, even when the total number of nematodes was different by a factor of more than ten. However, recovery rates varied between batches of roots collected at different times. Rate of recovery was not significantly affected by root sample size in the range 1–9 g
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An erratum to this article is available at http://dx.doi.org/10.1007/BF03215868.
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Moore, K.J., Southwell, R.J., Schwinghamer, M.W. et al. A rapid shake-elution procedure for quantifying root lesion nematodes (Pratylenchus thornei) in chickpea and wheat. Australasian Plant Pathology 21, 70–78 (1992). https://doi.org/10.1071/APP9920070
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DOI: https://doi.org/10.1071/APP9920070