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A Petri plate technique for testing pathogenicity of fungi to seedlings and inducing fungal sporulation

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Abstract

A Petri plate test is described which can be used to screen for pathogenicity of fungi from diseased seedlings, plant roots, seeds or soil. Seedlings grown axenically on water agar are inoculated with test fungi and infection observed directly. The test method is also valuable for fungal identification because it often promotes formation of fruiting structures which are not normally produced on conventional media. Sealed plates can be used for long-term storage of fungal cultures.

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References

  • Christensen, M.J. and Falloon, R.E. (1985)—Fungi pathogenic to forage brassica seedlings. Abstracts, 5th Australasian Plant Pathology Society Conference, Auckland, New Zealand, p. 25.

  • Falloon, R.E., Banfield, R.A. and Christensen, M.J. (1988) —Seedling disease of forage brassicas. Field Crops Research (in press).

  • Skipp, R.A. and Christensen, MJ. (1985)—Fungal invaders of ryegrass and white clover roots: comparison of host specificity in pasture soils and in vitro. Abstracts, 29th New Zealand Microbiological Society Conference, Palmerston North, p.88.

  • Skipp, R.A., Christensen, M.J. and Nan, Z.B. (1986) —Invasion of red clover (Trifolium pratense) roots by soilborne fungi. New Zealand Journal of Agricultural Research 29: 305–313.

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Christensen, M.J., Falloon, R.E. & Sklpp, R.A. A Petri plate technique for testing pathogenicity of fungi to seedlings and inducing fungal sporulation. Australasian Plant Pathology 17, 45–47 (1988). https://doi.org/10.1071/APP9880045

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  • DOI: https://doi.org/10.1071/APP9880045

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