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A modified method for clearing and staining leaf-infecting fungi in whole leaves

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Abstract

The dye Chlorazol Black E, incorporated in a leaf-clearing solution containing ethanol, chloroform, lactic acid, phenol and chloral hydrate, gave excellent differentiation of rust and powdery mildew infections in leaf tissue at room temperature.

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References

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Authors and Affiliations

  1. Department of Botany, La Trobe University, 3083, Bundoora, Victoria

    P. J. Keane, N. Limongiello & M. A. Warren

Authors
  1. P. J. Keane
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  2. N. Limongiello
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  3. M. A. Warren
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Keane, P.J., Limongiello, N. & Warren, M.A. A modified method for clearing and staining leaf-infecting fungi in whole leaves. Australasian Plant Pathology 17, 37–38 (1988). https://doi.org/10.1071/APP9880037

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  • DOI: https://doi.org/10.1071/APP9880037

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