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Histopathological Technique for Detection of Fungal Infections in Plants

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Laboratory Protocols in Fungal Biology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Microscopic examination of the interaction between pathogenic fungi and their host plants has been instrumental in deciphering the biology of this relationship and can serve as a useful diagnostic tool. In this chapter, we describe the technique of fixing fungal infections of plant samplings for histopathological experiments. Toluidine blue O’ staining methods coupled with stereoscopic microscopy are used to scan the infection structures of the fungus Fusarium spp. and host response in Psidium guajava L. root tissues.

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References

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Acknowledgements

The authors are very grateful to Director, CISH; Head, Department of Crop-Protection, Central Institute of Subtropical Horticulture (CISH), Lucknow; and Prof. Shakti Baijal, Ex-Dean, FASC, MITS University, Rajasthan for providing the necessary research grants.

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Authors and Affiliations

  1. Molecular Glycobiotechnology Group, Department of Biochemistry, School of Natural Sciences, National University of Ireland Galway, University Road, Galway, Ireland

    Vijai Kumar Gupta (Assistant Professor of Biotechnology)

  2. Department of Science, Faculty of Arts, Science & Commerce, MITS University, Sikar, Rajasthan, India

    Vijai Kumar Gupta (Assistant Professor of Biotechnology)

  3. Molecular Plant Pathology Laboratory, Central Institute for Subtropical Horticulture, Indian Council of Agricultural Research, P.O.kakori, Rahmankhera, Lucknow, UP, 227 017, India

    Brejesh Kumar Pandey

Authors
  1. Vijai Kumar Gupta
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  2. Brejesh Kumar Pandey
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Corresponding author

Correspondence to Vijai Kumar Gupta .

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Editors and Affiliations

  1. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Vijai Kumar Gupta

  2. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Maria G. Tuohy

  3. , Department of Biochemistry, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Manimaran Ayyachamy

  4. , Manufacturing Sciences and Technology, Pfizer Ireland Pharmaceuticals, The Pfizer Biotech Campus at Grange Castle, Clondalkin, N/A, Dublin, Ireland

    Kevin M. Turner

  5. , School of Natural Sciences, National University of Ireland Galway, University Road, Galway, N/A, Ireland

    Anthonia O’Donovan

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Gupta, V.K., Pandey, B.K. (2013). Histopathological Technique for Detection of Fungal Infections in Plants. In: Gupta, V., Tuohy, M., Ayyachamy, M., Turner, K., O’Donovan, A. (eds) Laboratory Protocols in Fungal Biology. Fungal Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-2356-0_13

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