Abstract
Selective isolation from soil of mycoparasites of Sclerotinia minor by baiting with mature cultures of the pathogen was compared with the use of a semi-selective, antibiotic-containing medium. Baiting was useful for selectively isolating and determining the concentration in soil of a potential biocontrol agent such as Trichoderma virens. The method proved to be useful for enumerating T: virens on roots of sunflower and showed promise for studies of the ecology of biocontrol agents added to soil or seed.
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Isnaini, M., Burgess, D.R. & Keane, P.J. The use of cultures of Sclerotinia minor for selective isolation and enumeration of mycoparasitic isolates of Trichoderma from soil and roots. Australasian Plant Pathology 27, 244–250 (1998). https://doi.org/10.1071/AP98027
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DOI: https://doi.org/10.1071/AP98027