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Molecular variation among isolates belonging to eight races of Phytophthora clandestina

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Abstract

Phytophthora clandestina, a serious pathogen of subterranean clover (Trifolium subterraneum), has only been recorded in Australia. A rapid method was used to characterise genetic variation among 61 isolates of P. clandestina belonging to eight pathogenic races based on DNA sequencing and single-strand conformation polymorphism (SSCP) analyses of the β-tubulin gene of the pathogen. The β-tubulin gene among those tested displayed a high degree of variability among isolates. Cluster analysis of the SSCP profiles grouped the 61 isolates into two main clusters (A and B). ClusterAwith 3 subclusters viz; I (race 177), II (race 000) and III (races 001, 121, 101, 143 and 157) and B with race 173 alone. In addition, SSCP of β-tubulin also successfully differentiated between races 173 and 177, the two most prevalent and most virulent of the races studied. This is the first time that the β-tubulin gene has been used to study intra-species variation in this pathogen. In addition to showing relationship among the strains, it also provides a practical means for rapid monitoring of current and future differences in the distribution of P. clandestina strains, giving subterranean clover breeders and farmers a sound basis for the selection/breeding and deployment of appropriate cultivars to counter the predominant strain populations in specific localities.

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Authors and Affiliations

  1. School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 6009, Crawley, WA, Australia

    X. L. Ma, Hua Li, K. Sivasithamparam & M. J. Barbetti

  2. Department of Plant Pathology and Weed Sciences, Virginia Polytechnic Institute and State University, Hampton Roads Agricultural Research and Extension Centre, 1444 Diamond Springs Road, 23455, Virginia Beach, VA, USA

    P. Kong

  3. Department of Agriculture and Food Western Australia, Baron-Hay Court, 6151, South Perth, WA, Australia

    M. P. You & M. J. Barbetti

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  1. X. L. Ma
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  2. P. Kong
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  3. M. P. You
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  4. Hua Li
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  5. K. Sivasithamparam
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  6. M. J. Barbetti
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Ma, X.L., Kong, P., You, M.P. et al. Molecular variation among isolates belonging to eight races of Phytophthora clandestina . Australasian Plant Pathology 38, 608–616 (2009). https://doi.org/10.1071/AP09047

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  • DOI: https://doi.org/10.1071/AP09047

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