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A nucleotide substitution in one of the β-tubulin genes of Trichoderma viride confers resistance to the antimitotic drug methyl benzimidazole-2-yl-carbamate

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Abstract

We characterized a Trichoderma viride strain that is resistant to the antimitotic drug methyl benzimidazole-2-yl-carbamate (MBC). This species has two β-tubulin genes (tub1 and tub2) and by reverse genetics we showed that a mutation in the tub2 gene confers MBC resistance in this strain. Comparison of the tub2 sequence of the mutant strain with that of the wild type revealed that a single amino acid substitution of tyrosine for histidine at position 6 is responsible for the MBC tolerance. Furthermore, we showed that this gene can be used as a homologous dominant selectable marker in T. viride transformation. Both tubulin genes were completely sequenced. They differ by 48 residues and the degree of identity between their deduced amino acid sequences is 86.3%.

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Author notes

  1. G. H. Goldman

    Present address: Department of Pharmacology, UMDNJ-Robert Wood Johnson Medical School, 08854, Piscataway, NJ, USA

  2. A. Herrera-Estrella

    Present address: Centro de Investigacion y Estudios Avanzados, Unidad Irapuato, A.P. 629, Irapuato, Mexico

Authors and Affiliations

  1. Laboratorium voor Genetica, Universiteit Gent, B-9000, Gent, Belgium

    G. H. Goldman, W. Temmerman, D. Jacobs, M. Van Montagu & A. Herrera-Estrella

  2. Laboratorium voor Moleculaire Biologie, Universiteit Gent, B-9000, Gent, Belgium

    R. Contreras

Authors
  1. G. H. Goldman
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  2. W. Temmerman
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  3. D. Jacobs
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  4. R. Contreras
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  5. M. Van Montagu
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  6. A. Herrera-Estrella
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Communicated by C.A.M.J.J. van den Hondel

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Goldman, G.H., Temmerman, W., Jacobs, D. et al. A nucleotide substitution in one of the β-tubulin genes of Trichoderma viride confers resistance to the antimitotic drug methyl benzimidazole-2-yl-carbamate. Molec. Gen. Genet. 240, 73–80 (1993). https://doi.org/10.1007/BF00276886

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  • DOI: https://doi.org/10.1007/BF00276886

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