Abstract
Three species of Mycosphaerella are known to cause Sigatoka leaf diseases in banana. M. musicola causes yellow Sigatoka, M. fijiensis causes black Sigatoka and M. eumusae causes eumusae leaf spot. Linie is known about the presence of Sigatoka leaf diseases of banana in South Africa. Although yellow Sigatoka has previously been reported, the identity of the causal organism has never been confirmed. Extensive surveys of the five banana-growing areas in South Africa were conducted between 1999 and 2001, which resulted in the collection of various Sigatoka-like leaf spot samples. After morphological examination of the infected material, monoconidial isolates of the causal organism were established from each sample. A molecular confirmation of identity was conducted using species-specific primers for M. musicola and M. fijiensis. Sequence data of the ITS region was further obtained to compare the South African Mycosphaerella isolates from banana with M. musicola, M. fijiensis and M. eumusae. These results confirm that only M. musicola is presently associated with yellow Sigatoka symptoms in South Africa.
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References
Carlier J, Mourichon X, Gonzalez-de-Leon D, Zapater MF, Lebrun, MH (1994) DNA Restriction fragment length polymorphisms in Mycosphaerella species that cause banana leaf spot diseases. Phytopathology 84, 751–756.
Carlier J, Zapater MF, Lapeyre F, Jones DR, Mourichon X (2000) Septoria leaf spot of banana: a newly discovered disease caused by Mycosphaerella eumusae (Anamorph Septoria eumusae). Phytopathology 90, 884–890.
Crous PW, Mourichon X (2002) Mycosphaerella eumusae and its anamorph Pseudocercospora eumusae sp. nov.: causal agent of eumusae leaf spot disease of banana. Sydowia 54, 35–43.
Johanson A, Jeger MJ (1993) Use of PCR for detection of Mycosphaerella fijiensis and M. musicola, the causal agents of Sigatoka leaf spots in banana and plantain. Mycological Research 96, 670–674.
Jones DR (2000) Fungal diseases of the foliage. In ‘Disease of banana, abaca and enset’. (Ed DR Jones) pp. 37–141. (CABI Publishing: Wallingford)
Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1, 17–20.
Stewart EL, Liu Z, Crous PW, Szabo LJ (1999) Phylogenetic relationships among some cercosperoid anamorphs of Mycosphaerella based on rDNA sequence analysis. Mycological Research 103, 1491–1499.
Stover RH (1972) Fungus diseases of the foliage. In ‘Banana, plantain and abaca diseases’. (Commonwealth Mycological Institute: Kew, Surrey)
Swofford DL (2000) Sinauer Associates ‘Phylogenetic Analysis Using Parsimony’, Version 4.0b8. (Sunderland, Massachusetts, USA)
Van den Boom T, Kuhne FA (1969) First report of Sigatoka disease of banana in South Africa. Citrus Journal 428, 17–18.
White TJ, Bruns T, Lee S, Taylor J (1990) PCR protocols. In ‘Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics’. (Eds MA Innis, DH Gelfand, JJ Sninsky, TJ White) pp. 315–322. (Academie Press: London)
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Surridge, A.K.J., Viljoen, A., Crous, R.W. et al. Identification of the pathogen associated with Sigatoka disease of banana in South Africa. Australasian Plant Pathology 32, 27–31 (2003). https://doi.org/10.1071/AP02058
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DOI: https://doi.org/10.1071/AP02058