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Using SSR markers to study the mechanism of 2n pollen formation in Populus × euramericana (Dode) Guinier and P. × popularis

Utilisation de marqueurs SSR pour étudier le mécanisme de formation du pollen 2n chez Populus × euramericana (Dode) Guinier et P. × popularis

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Abstract

  • • Using cytological methods and SSR DNA marker analysis, this study revealed the formation mechanisms and the genetic constitutions of the 2n pollen in Populus × euramericana (Dode) Guinier and P. × popularis.

  • • In P. × euramericana (Dode) Guinier, four abnormalities in microsporogenesis were observed: parallel spindle, fused spindle, tripolar spindle and premature cytokinesis. The first three can lead to first-division restitution (FDR) 2n pollen formation and the last one can form second-division restitution (SDR) 2n pollen. The SSR marker analysis results of parents and their tetraploidy filial generation confirmed that the genetic constitution of 2n pollen produced by P. × euramericana (Dode) Guinier was FDR.

  • • In P. × popularis, three of these abnormalities were observed: parallel spindle, fused spindle and premature cytokinesis. The SSR marker analysis results showed the genetic constitution of 2n pollen produced by P. × popularis was SDR. Natural 2n female gametes in P. × euramericana (Dode) Guinier are reported for the first time. SSR analysis indicated that natural 2n female gametes of P. × euramericana (Dode) Guinier did exist and were fertile, which could be FDR genetic constitution.

  • • The results from this study showed a great potential for using 2n gametes to produce polyploid poplar clones, which can be used effectively for polyploid breeding for poplar species in the section Aigeiros.

Résumé

  • • En utilisant des méthodes cytologiques et des analyses avec des marqueurs SSR, cette étude a révélé les mécanismes de formation et la constitution génétique du pollen 2n chez Populus × euramericana (Dode) Guinier et P. × popularis.

  • • Chez P. × euramericana (Dode) Guinier, quatre anomalies dans la microsporogénèse ont été observées : en parallèle broche, broche fusion,tripolaire broche et une cytokinèse prématurée. Les trois premiers peuvent entraîner une première division restitution (FDR) de formation du pollen 2n et le dernier peut former une deuxième division de restitution (SDR) du pollen 2n. Les résultats de l’analyse des marqueurs SSR des parents et de leur descendanta tetraploides ont confirmé que la constitution génétique du pollen 2n produit par P. × euramericana (Dode) Guinier a été FDR.

  • • Dans P. × popularis, trois de ces anomalies ont été observées : en parallèle broche, broche fusion et cytokinèse prématurée. Les résultats de l’analyse de marqueurs SSR ont montré que la constitution génétique du pollen 2n produit par P. × popularis a été SDR. Les gamètes naturelles femelles 2n chez P. × euramericana (Dode) Guinier ont été signalés pour la première fois. Les analyses SSR ont montré que les gamètes naturels femelles 2n de P. × euramericana (Dode) Guinier existaient et étaient fertiles, cela pourrait être la constitution génétique FDR.

  • • Les résultats de cette étude ont montré le grand potentiel de l’utilisation des gamètes 2n pour produire des clones de peuplier polyploïdes, cela peut être utilisé efficacement pour la sélection de polyploïdes pour des espèces de peuplier de la section Aigeiros.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, Beijing Forestry University, 100083, Beijing, P.R. China

    Jin-Feng Zhang, Zun-Zheng Wei, Dan Li & Bailian Li

  2. Key Laboratory of Genetic and Breeding of Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, 100083, Beijing, P.R. China

    Jin-Feng Zhang, Zun-Zheng Wei, Dan Li & Bailian Li

  3. Department of Forestry and Environmental Resources, North Carolina State University, 27695, Raleigh, North Carolina, USA

    Bailian Li

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  1. Jin-Feng Zhang
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  2. Zun-Zheng Wei
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  3. Dan Li
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  4. Bailian Li
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Correspondence to Jin-Feng Zhang or Bailian Li.

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Zhang, JF., Wei, ZZ., Li, D. et al. Using SSR markers to study the mechanism of 2n pollen formation in Populus × euramericana (Dode) Guinier and P. × popularis . Ann. For. Sci. 66, 506 (2009). https://doi.org/10.1051/forest/2009032

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  • DOI: https://doi.org/10.1051/forest/2009032

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