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A scientific note on a rapid method for the molecular discrimination of Apis andreniformis and A. florea

Note scientifique sur une méthode rapide de discrimination moléculaire d’Apis andreniformis et d’A. florea

Eine wissenschaftliche Bemerkung über eine schnelle Methode der molekularen Unterscheidung zwischen Apis andreniformis und A. florea

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Conclusion

Our results demonstrate that the size polymorphism in this fragment of the rrnL gene reliably distinguishes A. andreniformis and A. florea. With some slight modifications to the DNA extraction protocol, we have successfully employed the above method to assign species to adult drones, a queen and drone brood of all stages.

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References

  • Cameron S.A. (1993) Multiple origins of advanced eusociality in bees inferred from mitochondrial DNA sequences, Proc. Natl Acad. Sci. (USA) 90, 8687–8691.

    Article  CAS  Google Scholar 

  • Crozier R.H., Crozier Y.C. (1993) The mitochondrial genome of the honeybee Apis mellifera: Complete sequence and genome organization, Genetics 133, 97–117.

    PubMed  CAS  Google Scholar 

  • Fabricius J.C. (1787) Mantissa Insectorium Sistems eorum Species nuper Detectas adiectis Characteribus Genericus, Differentiis Specificis, Emendationibus, Observationibus, C.G. Proft, Copenhagen.

    Google Scholar 

  • Higgs J.S., Wattanachaiyingcharoen W., Oldroyd B.P. (2009) A scientific note on a genetically-determined color morph of the dwarf honey bee, Apis andreniformis, Apidologie 40, 513–514.

    Article  Google Scholar 

  • Maa T. (1953) An inquiry into the systematics of the tribus Apidini or honeybees (Hym.), Treubia 21, 525–640.

    Google Scholar 

  • Oldroyd B.P., Wongsiri S. (2006) Introduction to the species, in: Asian honey bees: Biology, conservation, and human interactions, Harvard University Press, Cambridge, pp. 13–35.

    Google Scholar 

  • Oldroyd B.P., Clifton M.J., Parker K., Wongsiri S., Rinderer T.E., Crozier R.H. (1998) Evolution of mating behavior in the genus Apis and an estimate of mating frequency in Apis cerana (Hymenoptera: Apidae), Ann. Entomol. Soc. Am. 91, 700–709.

    CAS  Google Scholar 

  • Raffiudin R., Crozier R.H. (2007) Phylogenetic analysis of honey bee behavioral evolution, Mol. Phylogenet. Evol. 43, 543–552.

    Article  PubMed  CAS  Google Scholar 

  • Smith D.R. (1991) Mitochondrial DNA and honey bee biogeography, in: Diversity in the genus Apis, Westview Press, Boulder, pp. 131–176.

    Google Scholar 

  • Smith F. (1858) Catalogue of hymenopterous insects collected Sarawak, Borneo; Mount Ophir, Malacca; and at Singapore, in: Wallace A.R. (Ed.), Proc. Linn. Soc. Lond. 2, 42–130.

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Authors and Affiliations

  1. Behaviour and Genetics of Social Insects Lab, School of Biological Sciences A12, University of Sydney, 2006, NSW, Australia

    Jessica S. Higgs, Marcus McHale & Benjamin P. Oldroyd

Authors
  1. Jessica S. Higgs
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  2. Marcus McHale
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  3. Benjamin P. Oldroyd
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Correspondence to Benjamin P. Oldroyd.

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Manuscript editor: Steve Sheppard

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Higgs, J.S., McHale, M. & Oldroyd, B.P. A scientific note on a rapid method for the molecular discrimination of Apis andreniformis and A. florea . Apidologie 41, 96–98 (2010). https://doi.org/10.1051/apido/2009060

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  • DOI: https://doi.org/10.1051/apido/2009060

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