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A long-wavelength ultrasensitive colorimetric fluorescent probe for carbon monoxide detection in living cells

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Abstract

Exploring techniques for monitoring the intracellular signaling molecule carbon monoxide (CO) in biosystems is important to help understand its various cellular functions. Therefore, a simple long-wavelength colorimetric fluorescent probe LW-CO was designed for selectively and sensitively detecting intracellular CO in living systems. Probe LW-CO is ultrasensitive and can track CO levels in the range of 0–1 μM, with a detection limit of about 3.2 nM. Additionally, the obvious color changes of probe LW-CO with CO (yellow to pink) provide a convenient way for on-site detection of CO with the naked eye. Probe LW-CO was applied to track the exogenous levels of CO in RAW264.7 cells. Probe LW-CO proved to be an efficient method for investigating various cellular functions of CO.

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Authors and Affiliations

  1. School of Water Conservancy and Environment, University of Jinan, Shandong Provincial Engineering Technology Research Center for Ecological Carbon Sink and Capture Utilization, Jinan, 250022, China

    Zuokai Wang, Ziyang Zhao, Caiyun Liu, Zhuofan Geng, Qingxia Duan, Pan Jia, Zilu Li, Hanchuang Zhu & Baocun Zhu

  2. Qilu University of Technology (Shandong Academy of Sciences), Biology Institute of Shandong Academy of Sciences, 19 Keyuan Road, Lixia District, Jinan, Shandong Province, 250014, China

    Wenlong Sheng

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  1. Zuokai Wang
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  2. Ziyang Zhao
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  3. Caiyun Liu
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  4. Zhuofan Geng
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  5. Qingxia Duan
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  6. Pan Jia
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  7. Zilu Li
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  8. Hanchuang Zhu
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  9. Baocun Zhu
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  10. Wenlong Sheng
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Correspondence to Baocun Zhu.

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Electronic supplementary information (ESI) available. See DOI: 10.1039/c9pp00222g

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Wang, Z., Zhao, Z., Liu, C. et al. A long-wavelength ultrasensitive colorimetric fluorescent probe for carbon monoxide detection in living cells. Photochem Photobiol Sci 18, 1851–1857 (2019). https://doi.org/10.1039/c9pp00222g

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